SDS-Page
GAGA-POZ domain Recombinant Protein | GAGA-POZ recombinant protein
GAGA-POZ domain, 1-130aa, Drosophila melanogaster, E Coli
Gene Names
Trl; Adf-2; Adf-2-519; Adf2; anon-EST:fe2E12; CG33261; CG9343; DmelCG33261; E(var)3-trl; E(var)62; Gaf; GAF; gaga; Gaga; GAGA; GAGA factor; l(3)s2325; Nc70F; NC70F; TfGAGA/Adf-2; trl; TRL; Trl-GAGA
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
GAGA-POZ domain; 1-130aa; Drosophila melanogaster; E Coli; Trithorax-like isoform A; Trl; Adf-2; GAGA; Nc70F; TFGAGA; Trithorax-like protein; GAGA factor; GAF; Adh transcription factor 2; Neural conserved at 70F; Transcription factor GAGA; Trithorax like protein; GAGA-POZ recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 10 mM HEPES (pH 7.4), 25mM NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH
Sequence Length
582
Applicable Applications for GAGA-POZ recombinant protein
SDS-PAGE
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for GAGA-POZ recombinant protein
The GAGA factor is a sequence-specific DNA-binding protein, which participates in the regulation of the expression of a variety of different classes of genes in Drosophila such as many developmentally regulated genes, stress induced genes, and cell cycle regulated genes, as well as housekeeping genes. GAGA contains a C-terminal glutamine-rich domain and a highly conserved N-terminal POZ domain which reported to be involved in self-oligomerization in a number of other POZ domain containing proteins. In case of GAGA protein, the N-terminal POZ domain mediates the formation of oligomers both in vitro and in vivo. GAGA-POZ domain was overexpressed in E Coli and purified by using conventional chromatography techniques
Product Categories/Family for GAGA-POZ recombinant protein
References
Kosoy A., et al. (2002) J Biol.Chem. 277 (44), 42280-8.; Espinas ML., et al. (1999) J Biol. Chem. 274 (23),16461-9.; Farkas G., et al. (1994) Nature 371, 806-808.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14 kDa (130 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
Trithorax-like, isoform A
NCBI Official Synonym Full Names
Trithorax-like
NCBI Official Symbol
Trl
NCBI Official Synonym Symbols
Adf-2; Adf-2-519; Adf2; anon-EST:fe2E12; CG33261; CG9343; DmelCG33261; E(var)3-trl; E(var)62; Gaf; GAF; gaga; Gaga; GAGA; GAGA factor; l(3)s2325; Nc70F; NC70F; TfGAGA/Adf-2; trl; TRL; Trl-GAGA
NCBI Protein Information
CG33261 gene product from transcript CG33261-RI
UniProt Protein Name
Transcription factor GAGA
UniProt Gene Name
Trl
UniProt Synonym Gene Names
Adf-2; GAGA; Nc70F; TFGAGA; GAF
Uniprot Description
Transcriptional activator that functions by regulating chromatin structure. Overcomes the repressive effects of chromatin by promoting the open chromatin conformation in promoter gene regions, thereby allowing access to other transcription factors. Binds to DNA Polycomb response elements (PREs) at the bithorax complex and to the proximal region of the engrailed promoter, and positively regulates transcription of many genes including homeotic ones. Binds to the DNA sequence (GA)n, with optimal binding to the pentamer 5'-GAGAG-3'. Binds DNA as an oligomer. May also act as a transcriptional repressor, maintaining the repressed state of genes including lolal, and down-regulating its own transcription. Required for dosage compensation in males and may be involved in oogenesis. Also has a role in nuclear division.
Research Articles on GAGA-POZ
Similar Products
Product Notes
The GAGA-POZ trl (Catalog #AAA203061) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GAGA-POZ domain can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GAGA-POZ trl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSLPMNSLYS LTWGDYGTSL VSAIQLLRCH GDLVDCTLAA GGRSFPAHKI VLCAASPFLL DLLKNTPCKH PVVMLAGVNA NDLEALLEFV YRGEVSVDHA QLPSLLQAAQ CLNIQGLAPQ TVTKDDYTTH. It is sometimes possible for the material contained within the vial of "GAGA-POZ domain, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
