Rabbit anti-Human MYF5 Polyclonal Antibody | anti-MYF5 antibody

MYF5, NT (MYF5, BHLHC2, Myogenic factor 5, Class C basic helix-loop-helix protein 2) (APC)

Cat. Num. AAA6323265

• Gene Names: MYF5; bHLHc2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MYF5; Polyclonal Antibody; NT (MYF5; BHLHC2; Myogenic factor 5; Class C basic helix-loop-helix protein 2) (APC); Class C basic helix-loop-helix protein 2; anti-MYF5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MYF5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MYF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-36 amino acids from the N-terminal region of human MYF5.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MYF5 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the MYF5 antibody detected the MYF5 protein (arrow).)

Related Product Information for anti-MYF5 antibody

MYF5 is involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Product Categories/Family for anti-MYF5 antibody

Antibodies; Transcription Factors

References

Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Venza, M., et al. J. Oral Pathol. Med. 38(1):18-23(2009). Ansseau, E., et al. PLoS ONE 4 (10), E7482 (2009): Kosek, D.J., et al. J. Appl. Physiol. 101(2):531-544(2006). Summerbell, D., et al. Mech. Dev. 117 (1-2), 331-335 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 156104906
• NCBI GeneID: 4617
• NCBI Accession #: NP_005584.2
• NCBI GenBank Nucleotide #: NM_005593.2
• UniProt Accession #: P13349; Q6ISR9
• Molecular Weight: 28,296 Da
• NCBI Official Full Name: myogenic factor 5
• NCBI Official Synonym Full Names: myogenic factor 5
• NCBI Official Symbol: MYF5
• NCBI Official Synonym Symbols: bHLHc2
• NCBI Protein Information: myogenic factor 5; myf-5; class C basic helix-loop-helix protein 2
• UniProt Protein Name: Myogenic factor 5
• Protein Family: Myogenic factor
• UniProt Gene Name: MYF5
• UniProt Synonym Gene Names: BHLHC2; Myf-5; bHLHc2
• UniProt Entry Name: MYF5_HUMAN

Uniprot Description

Myf-5: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: nucleoplasm

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity

Biological Process: transcription from RNA polymerase II promoter; ossification; extracellular matrix organization and biogenesis; muscle development; skeletal muscle development; somitogenesis; camera-type eye development; regulation of cell-matrix adhesion; muscle cell fate commitment; positive regulation of myoblast differentiation; embryonic skeletal morphogenesis; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; cartilage condensation