SSA1, Western Blot Control Recombinant Protein | TRIM21 recombinant protein

SSA1, Recombinant, Human, Western Blot Control (Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

Cat. Num. AAA635771

• Gene Names: TRIM21; SSA; RO52; SSA1; RNF81
• Applications: Western Blot
• Purity: Purified; 0.95
• Synonyms: SSA1; Western Blot Control; Recombinant; Human; Western Blot Control (Sjogren Syndrome Antigen A1; 52kD Ribonucleoprotein Autoantigen SS-A/Ro; RO52; RNF81; Tripartite Motif Protein 21; TRIM21); TRIM21 recombinant protein

• Purity/Purification: Purified; 0.95
• Form/Format: Supplied as a liquid in SDS-PAGE sample buffer (reduced).

• Applicable Applications for TRIM21 recombinant protein: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: Load 10ul/lane.; Optimal dilution to be determined by researcher
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for TRIM21 recombinant protein

Molecular Biology; MB-Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 14994115
• NCBI GeneID: 6737
• UniProt Accession #: P19474; Q5XPV5; Q96RF8
• Molecular Weight: 54,170 Da
• NCBI Official Full Name: SSA1
• NCBI Official Synonym Full Names: tripartite motif containing 21
• NCBI Official Symbol: TRIM21
• NCBI Official Synonym Symbols: SSA; RO52; SSA1; RNF81
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM21; SS-A; ro(SS-A); 52 kDa Ro protein; RING finger protein 81; Sicca syndrome antigen A; tripartite motif-containing 21; sjoegren syndrome type A antigen; tripartite motif-containing protein 21; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM21
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM21
• UniProt Synonym Gene Names: RNF81; RO52; SSA1; SS-A
• UniProt Entry Name: RO52_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ('Thr- 187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ligase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; cytoplasm; SCF ubiquitin ligase complex; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: protein monoubiquitination; regulation of interferon type I production; negative regulation of viral transcription; protein autoubiquitination; protein polyubiquitination; positive regulation of virion penetration into host cell; protein destabilization; inhibition of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; positive regulation of interferon type I production; protein ubiquitination; positive regulation of cell cycle; innate immune response; positive regulation of transcription factor activity; cell cycle