TPST1 recombinant protein

TPST1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120763

• Gene Names: TPST1; TANGO13A
• Purity: >80% as determined by SDS-PAGE
• Synonyms: TPST1; TPST1 Protein; Human; Recombinant (His Tag); Human TPST1 Protein (His Tag); TANGO13A Protein; tyrosylprotein sulfotransferase 1; TPST1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln26-Glu370

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human TPST1 (NP_003587.1) extracellular domain (Gln 26-Glu 370) was expressed, with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TPST1 recombinant protein

Background: Protein-tyrosine sulfotransferase 1, also known as Tyrosylprotein sulfotransferase 1 and TPST1, is a single-pass type II membrane protein that belongs to the protein sulfotransferase family. Tyrosine O-sulfation is a common posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called tyrosylprotein sulfotransferase (TPST) that catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to tyrosine residues within acidic motifs of polypeptides. Tyrosine O-sulfation has been shown to be important in protein-protein interactions in several systems. Tyrosine sulfation is mediated by one of two Golgi isoenzymes, called tyrosylprotein sulfotransferases (TPST-1 and TPST-2). A relatively small number of proteins are known to undergo tyrosine sulfation, including certain adhesion molecules, G-protein-coupled receptors, coagulation factors, serpins, extracellular matrix proteins, and hormones. TPST1 is a human tyrosylprotein sulfotransferase that uses 3'phosphoadenosine-5'phosphosulfate (PAPS) to transfer the sulfate moiety to proteins predominantly designated for secretion. TPST1 bears N-linked glycosyl residues exclusively at position Asn6 and Asn262. TPST1 and TPST2 have distinct biological roles that may reflect differences in their macromolecular substrate specificity.

References

Ouyang Y.-B.et al., 1998, Proc. Natl. Acad. Sci. USA. 95: 2896-901. Ouyang,Y.B. et al., 2002,J Biol Chem. 277 (26):23781-7. Hoffhines, A.J. et al., 2006, J Biol Chem. 281 (49):37877-87. Goettsch,S. et al., 2006, J Mol Biol. 361 (3):436-49. Westmuckett, A.D. et al., 2008, Gen Comp Endocrinol. 156 (1):145-53.

NCBI and Uniprot Product Information

• NCBI GI #: 4507665
• NCBI GeneID: 8460
• NCBI Accession #: NP_003587.1
• NCBI GenBank Nucleotide #: NM_003596.3
• UniProt Accession #: O60507; Q6FGM7; A4D2M0
• Molecular Weight: 42,188 Da
• NCBI Official Full Name: protein-tyrosine sulfotransferase 1
• NCBI Official Synonym Full Names: tyrosylprotein sulfotransferase 1
• NCBI Official Symbol: TPST1
• NCBI Official Synonym Symbols: TANGO13A
• NCBI Protein Information: protein-tyrosine sulfotransferase 1
• UniProt Protein Name: Protein-tyrosine sulfotransferase 1
• Protein Family: Protein-tyrosine sulfotransferase
• UniProt Gene Name: TPST1
• UniProt Synonym Gene Names: TPST-1
• UniProt Entry Name: TPST1_HUMAN

Uniprot Description

TPST1: Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides. Belongs to the protein sulfotransferase family.

Protein type: EC 2.8.2.20; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 7q11.21

Cellular Component: Golgi apparatus; membrane

Biological Process: inflammatory response