Human Tryptase ELISA Kit | TPSAB1 elisa kit

Human Tryptase ELISA Kit

Cat. Num. AAA8248412

• Gene Names: TPSAB1; TPS1; TPS2; TPSB1
• Reactivity: Human
• Synonyms: Tryptase; Human Tryptase ELISA Kit; TPSAB1; TPS1; TPS2; TPSB1; Tryptase alpha/beta-1; Tryptase-1; Tryptase I; Tryptase alpha-1; TPSB2; Tryptase beta-2; Tryptase-2; Tryptase II; TPSAB1 elisa kit

• Reactivity: Human
• Specificity: The Human Tryptase ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Tryptase proteins within the range of 156 pg/ml - 10000 pg/ml.
• Sequence Length: 275

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <15 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TPSAB1 elisa kit

Principle of the Assay: The Human Tryptase ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Tryptase in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human Tryptase coated on a 96-well plate. Standards and samples are pipetted into the wells and Tryptase present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Tryptase antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Tryptase bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

NCBI and Uniprot Product Information

• NCBI GI #: 13775595
• NCBI GeneID: 7177
• NCBI Accession #: NP_003285.2
• NCBI GenBank Nucleotide #: NM_003294.3
• UniProt Accession #: Q15661; P15157; Q15663; Q6B052; Q9H2Y4; Q9H2Y5; Q9UQI1; D2E6R9; D2E6S1
• Molecular Weight: 29,533 Da
• NCBI Official Full Name: tryptase alpha/beta-1
• NCBI Official Synonym Full Names: tryptase alpha/beta 1
• NCBI Official Symbol: TPSAB1
• NCBI Official Synonym Symbols: TPS1; TPS2; TPSB1
• NCBI Protein Information: tryptase alpha/beta-1
• UniProt Protein Name: Tryptase alpha/beta-1
• Protein Family: Tryptase
• UniProt Gene Name: TPSAB1
• UniProt Synonym Gene Names: TPS1; TPS2; TPSB1; Tryptase-1
• UniProt Entry Name: TRYB1_HUMAN

NCBI Description

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.59; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: defense response; extracellular matrix disassembly