ELISA (EIA)
(Sandwich ELISA analysis of human TIMP-1 using Mouse anti Human TIMP-1 as a capture reagent and biotinylated Mouse anti Human TIMP-1(MBS226239) as a detection reagent with purified recombinant TIMP-1 as the antigen. Detection was perform)
Mouse anti-Human TIMP-1 Monoclonal Antibody | anti-TIMP-1 antibody
Mouse anti Human TIMP-1:Biotin
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human TIMP-1 using Mouse anti Human TIMP-1 as a capture reagent and biotinylated Mouse anti Human TIMP-1(MBS226239) as a detection reagent with purified recombinant TIMP-1 as the antigen. Detection was perform)
ELISA (EIA)
(Sandwich ELISA analysis of human TIMP-1 using Mouse anti Human TIMP-1 as a capture reagent and biotinylated Mouse anti Human TIMP-1(MBS226239) as a detection reagent with purified recombinant TIMP-1 as the antigen. Detection was perform)
TIMP-1 is a member of the TIMP family of proteins that assist in regulating the activity of matrix metalloproteinases (MMPs) and it is mainly expressed by macrophages and fibroblasts. TIMP proteins are specific endogenous inhibitors that bind to the active site of MMPs in a stoichiometric 1:1 molar ratio, thereby blocking access to ECM substrates (Kapoor et al. 2016, Robert et al. 2016). The TIMP proteins including TIMP-1 have poor specificity for any particular MMP and each can inhibit multiple MMPs with differing efficacies (Cui et al. 2017).
The ratio of MMP and TIMP often determine the extent of ECM protein degradation and tissue remodeling. An imbalance of this ratio such as with decreased expression of TIMP can result in various pathological conditions, most notably, heart failure, osteoarthritis and cancer. Low levels of TIMP have also been associated with slow-to-heal wounds and venous wounds (Cui et al. 2017).
The biotinylated Mouse anti Human TIMP-1 antibody, clone M05 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human TIMP-1 antibody, clone M03 as the capture antibody.
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008]
Uniprot Description
TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xp11.3-p11.23
Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region; basement membrane
Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; growth factor activity; metal ion binding; cytokine activity
Biological Process: response to peptide hormone stimulus; platelet activation; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis
Research Articles on TIMP-1
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Product Notes
The TIMP-1 timp1 (Catalog #AAA226239) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human TIMP-1:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIMP-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the TIMP-1 timp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIMP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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