Western Blot (WB)
(MyoD1 Antibody (Center) western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).)
Rabbit anti-Human MyoD1 Polyclonal Antibody | anti-MYOD1 antibody
MyoD1 Antibody (Center)
Western Blot (WB)
(MyoD1 Antibody (Center) western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).)
Western Blot (WB)
(MyoD1 Antibody (Center) western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with MyoD1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of Hela cell stained with MyoD1 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly.)
basic helix-loop-helix family of transcription factors and the
myogenic factors subfamily. It regulates muscle cell
differentiation by inducing cell cycle arrest, a prerequisite for
myogenic initiation. The protein is also involved in muscle
regeneration. It activates its own transcription which may
stabilize commitment to myogenesis.
Stuelsatz, P., et al. J. Biol. Chem. 285(17):12670-12683(2010)
Hiraoka, S., et al. Hum. Pathol. 41(1):38-47(2010)
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Yang, Z., et al. Genes Dev. 23(6):694-707(2009)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq, Jul 2008]
Uniprot Description
MyoD: a transcription factor of the basic helix-loop-helix family and the myogenic factors subfamily. Involved in muscle differentiation. Induces fibroblasts to differentiate into myoblasts. It is involved in muscle cell differentiation, and is essential for repair of damaged tissue. It activates its own transcription which may stabilize commitment to myogenesis.
Protein type: Transcription factor; DNA-binding; RNA splicing
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: nucleoplasm; transcription factor complex; myofibril; cytoplasm; nuclear chromatin; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein heterodimerization activity; chromatin DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; nuclear hormone receptor binding
Biological Process: transcription from RNA polymerase II promoter; muscle development; skeletal muscle development; skeletal muscle fiber adaptation; positive regulation of skeletal muscle regeneration; regulation of RNA splicing; myotube differentiation involved in skeletal muscle regeneration; muscle cell fate commitment; myotube cell development; skeletal muscle fiber development; cellular response to starvation; protein amino acid phosphorylation; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; muscle cell differentiation; positive regulation of skeletal muscle fiber development; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; myoblast cell fate determination; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on MYOD1
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Product Notes
The MYOD1 myod1 (Catalog #AAA9214241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 211-240. The MyoD1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MyoD1 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the MYOD1 myod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MyoD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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