TPP1 recombinant protein

TPP1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123227

• Gene Names: Tpp1; Cln2; LPIC; TPP-1; TPP-I
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TPP1; TPP1 Protein; Mouse; Recombinant (His Tag); Mouse TPP1/CLN2 Protein (His Tag); Cln2 Protein; LPIC Protein; TPP-1 Protein; TPP-I Protein; tripeptidyl peptidase I; TPP1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH7.5, 10% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro562

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Thr 20
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse TPP1 (NP_034036.1)(Met1-Pro562) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TPP1 recombinant protein

Background: Tripeptidyl-peptidase 1 (TPP1/CLN2) is a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. TPP1/CLN2 may act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Defects in TPP1/CLN2 are the cause of neuronal ceroid lipofuscinosis type 2 (CLN2), a form of neuronal ceroid lipofuscinosis which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy.

Product Categories/Family for TPP1 recombinant protein

Serine Proteases and Regulators

References

Xin H, et al. (2007) TPP1 is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase. Nature. 445(7127): 559-62.O'Connor MS, et al. (2006) A critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly. Proc Natl Acad Sci U S A. 103(32): 11874-9.Abreu E, et al. (2010) TIN2-tethered TPP1 recruits human telomerase to telomeres in vivo. Mol Cell Biol. 30(12): 2971-82.

NCBI and Uniprot Product Information

• NCBI GI #: 6753448
• NCBI GeneID: 12751
• NCBI Accession #: NP_034036.1
• NCBI GenBank Nucleotide #: NM_009906.6
• UniProt Accession #: O89023; Q543Q8; Q9QUS7
• Molecular Weight: 61,342 Da
• NCBI Official Full Name: tripeptidyl-peptidase 1 preproprotein
• NCBI Official Synonym Full Names: tripeptidyl peptidase I
• NCBI Official Symbol: Tpp1
• NCBI Official Synonym Symbols: Cln2; LPIC; TPP-1; TPP-I
• NCBI Protein Information: tripeptidyl-peptidase 1
• UniProt Protein Name: Tripeptidyl-peptidase 1
• Protein Family: Tripeptidyl-peptidase
• UniProt Gene Name: Tpp1
• UniProt Synonym Gene Names: Cln2; TPP-1; LPIC; TPP-I

NCBI Description

This gene encodes a lysosomal serine protease that cleaves N-terminal tripeptides from protein substrates. The encoded preproprotein undergoes autocatalytic processing to generate a mature enzyme. Mice lacking the encoded protein exhibit a progressive neurodegeneration and a greatly shortened lifespan. At the cellular level, mice lacking the encoded protein exhibit accumulation of autofluorescent lipopigments. Mutations in the human ortholog of this gene cause classical late-infantile neuronal ceroid lipofuscinosis. [provided by RefSeq, Nov 2015]

Uniprot Description

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.