Typical Testing Data/Standard Curve (for reference only)
Rat Anti-Thyroid peroxidase antibody ELISA Kit | TPO elisa kit
Rat Anti-Thyroid peroxidase antibody ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: TPAb ELISA kit applies the competitive enzyme immunoassay technique utilizing Thyroid Peroxidase antigen and an TPAb-HRP conjugate. The assay sample and buffer are incubated together with TPAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TPAb concentration since TPAb from samples and TPAb-HRP conjugate compete for the Thyroid Peroxidase antigen binding site. Since the number of sites is limited, as more sites are occupied by TPAb from the sample, fewer sites are left to bind TPAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPAb concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]
Uniprot Description
TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 1.11.1.8; Oxidoreductase; Mitochondrial; Amino Acid Metabolism - tyrosine
Chromosomal Location of Human Ortholog: 2p25
Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane
Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding
Biological Process: hydrogen peroxide catabolic process; embryonic hemopoiesis; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process
Disease: Thyroid Dyshormonogenesis 2a
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Product Notes
The Rat TPO (Catalog #AAA725714) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA725714 ELISA Kit recognizes Rat TPO. It is sometimes possible for the material contained within the vial of "Anti-Thyroid peroxidase antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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