TPMT recombinant protein

Recombinant Human TPMT protein

Cat. Num. AAA1562145

• Purity: >90% as determined by SDS-PAGE
• Synonyms: TPMT; Recombinant Human TPMT protein; Thiopurine S-methyltransferase; Thiopurine methyltransferase; TPMT recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human TPMT (Leu26-His227) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for TPMT recombinant protein

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor. TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.

Product Categories/Family for TPMT recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 1730006
• NCBI GeneID: 7172
• UniProt Accession #: P51580; O14806; O15423; O15424; O15425; O15426; O15515; O15548; O43213; Q5VUK6; Q9UBE6; Q9UBT8
• Molecular Weight: 245
• NCBI Official Full Name: Thiopurine S-methyltransferase
• NCBI Official Synonym Full Names: thiopurine S-methyltransferase
• NCBI Official Symbol: TPMT
• NCBI Protein Information: thiopurine S-methyltransferase; S-adenosyl-L-methionine:thiopurine S-methyltransferase
• UniProt Protein Name: Thiopurine S-methyltransferase
• UniProt Gene Name: TPMT
• UniProt Entry Name: TPMT_HUMAN

NCBI Description

This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]

Uniprot Description

TPMT: Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. Defects in TPMT are the cause of thiopurine S- methyltransferase deficiency (TPMT deficiency). TPMT is an enzyme involved in the normal metabolic inactivation of thiopurine drugs. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Patients with intermediate or no TPMT activity are at risk of toxicity after receiving standard doses of thiopurine drugs and it is shown that inter-individual differences in response to these drugs are largely determined by genetic variation at the TPMT locus. Belongs to the methyltransferase superfamily. TPMT family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.1.1.67; Methyltransferase

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: cytosol

Molecular Function: thiopurine S-methyltransferase activity

Biological Process: methylation; xenobiotic metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process

Disease: Thiopurine S-methyltransferase Deficiency