Human Tumor protein D52 ELISA Kit | TPD52 elisa kit

Human Tumor protein D52 ELISA Kit

Cat. Num. AAA765488

• Gene Names: TPD52; D52; N8L; PC-1; PrLZ; hD52
• Reactivity: Human
• Synonyms: Tumor protein D52; Human Tumor protein D52 ELISA Kit; TPD52/Tumor protein D52/Protein N8/D52/N8L/PC-1/PrLZ/hD52; TPD52 elisa kit

• Reactivity: Human
• Sequence Length: 184

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TPD52 elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPD52. During the reaction,Human TPD52 in the sample or standard competes with a fixed amount of biotin-labeled Human TPD52 for sites on a pre-coated Monoclonal antibody specific to Human TPD52. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human TPD52 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17390257
• NCBI GeneID: 7163
• Molecular Weight: 16,853 Da
• NCBI Official Full Name: Tumor protein D52
• NCBI Official Synonym Full Names: tumor protein D52
• NCBI Official Symbol: TPD52
• NCBI Official Synonym Symbols: D52; N8L; PC-1; PrLZ; hD52
• NCBI Protein Information: tumor protein D52
• UniProt Protein Name: Tumor protein D52
• Protein Family: Tumor protein
• UniProt Gene Name: TPD52
• UniProt Entry Name: TPD52_HUMAN

Uniprot Description

TPD52: a coiled-coil motif bearing hydrophilic protein known to be overexpressed in many human solid tumors of diverse cellular origins. May be associated with B cell differentiation. Increased expression is associated with increased proliferation and invasive capacity in different cell types. May negatively regulate ATM protein levels. Isoform 2 is expressed in colon, breast, prostate, pancreas and kidney tumor cell lines. Isoform 2 is expressed at high levels in kidney, prostate, brain, small intestine and pancreas, at moderate levels in placenta and colon, at low levels in lung, liver and heart, and at very low levels in spleen, thymus, peripheral mononuclear blood cells, testis and ovary. Isoform 2 is expressed at lower levels in fetal brain and kidney than in adult brain and kidney. Eight isoforms of the human protein are produced by alternative splicing

Protein type: Calcium-binding; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: cytoplasm; endoplasmic reticulum; perinuclear region of cytoplasm

Molecular Function: calcium ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: anatomical structure morphogenesis; B cell differentiation; secretion