TP53I3 recombinant protein

TP53I3, 1-332aa, Human, His tag, E Coli

Cat. Num. AAA203682

• Gene Names: TP53I3; PIG3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TP53I3; 1-332aa; Human; His tag; E Coli; Quinone oxidoreductase PIG3; PIG3; Quinone oxidoreductase PIG3 p53 induced gene 3 protein; Putative quinone oxidoreductase; quinone oxidoreductase homolog; Tumor protein p53 inducible protein 3.; TP53I3 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MLAVHFDKPG GPENLYVKEV AKPSPGEGEV LLKVAASALN RADLMQRQGQ YDPPPGASNI LGLEASGHVA ELGPGCQGHW KIGDTAMALL PGGGQAQYVT VPEGLLMPIP EGLTLTQAAA IPEAWLTAFQ LLHLVGNVQA GDYVLIHAGL SGVGTAAIQL TRMAGAIPLV TAGSQKKLQM AEKLGAAAGF NYKKEDFSEA TLKFTKGAGV NLILDCIGGS YWEKNVNCLA LDGRWVLYGL MGGGDINGPL FSKLLFKRGS LITSLLRSRD NKYKQMLVNA FTEQILPHFS TEGPQRLLPV LDRIYPVTEI QEAHKYMEAN KNIGKIVLEL PQ
• Sequence Length: 332

• Applicable Applications for TP53I3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TP53I3 recombinant protein

TP53I3, also known as quinone oxidoreductase PIG3, is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. It is localized to the cytoplasm and induced in primary, non-transformed and transformed cell cultures after exposure to genotoxic agents. This protein has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Recombinant human TP53I3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TP53I3 recombinant protein

Cancer

References

Flatt PM. et al. (2000) Cancer Lett. 156 (1):63-72.; Porte S, et al. (2009) J Biol Chem.. 284 (25):17194-205;

NCBI and Uniprot Product Information

• NCBI GI #: 22538446
• NCBI GeneID: 9540
• NCBI Accession #: NP_671713
• NCBI GenBank Nucleotide #: NM_147184.3
• UniProt Accession #: Q53FA7; O14679; O14685; Q38G78; Q6JLE7; Q9BWB8; D6W533
• Molecular Weight: 37.6 kDa (352aa), confirmed by MALDI-TOF
• NCBI Official Full Name: quinone oxidoreductase PIG3 isoform 1
• NCBI Official Synonym Full Names: tumor protein p53 inducible protein 3
• NCBI Official Symbol: TP53I3
• NCBI Official Synonym Symbols: PIG3
• NCBI Protein Information: quinone oxidoreductase PIG3
• UniProt Protein Name: Quinone oxidoreductase PIG3
• Protein Family: Quinone oxidoreductase
• UniProt Gene Name: TP53I3
• UniProt Synonym Gene Names: PIG3

NCBI Description

The protein encoded by this gene is similar to oxidoreductases, which are enzymes involved in cellular responses to oxidative stresses and irradiation. This gene is induced by the tumor suppressor p53 and is thought to be involved in p53-mediated cell death. It contains a p53 consensus binding site in its promoter region and a downstream pentanucleotide microsatellite sequence. P53 has been shown to transcriptionally activate this gene by interacting with the downstream pentanucleotide microsatellite sequence. The microsatellite is polymorphic, with a varying number of pentanucleotide repeats directly correlated with the extent of transcriptional activation by p53. It has been suggested that the microsatellite polymorphism may be associated with differential susceptibility to cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent beta-naphthoquinone reductase activity, with a preference for 1,2-beta-naphthoquinone over 1,4-beta-naphthoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).