Tobacco Etch Virus Protease Recombinant Protein | TEV recombinant protein
Recombinant Tobacco Etch Virus Protease
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Synonyms
Tobacco Etch Virus Protease; Recombinant Tobacco Etch Virus Protease; TEV; Tobacco Etch Virus Protease Recombinant; rTEV; P1 protease; TEV recombinant protein
Host
E Coli
Purity/Purification
Greater than 90.0% as determined by analysis by SDS-PAGE.
Form/Format
The rTEV contains 25mM Tris, 75mM NaCI, 5mM EDTA, 10mM GSH, 50% Glycerol.
Sequence Length
303
Physical Appearance
Sterile liquid formulation (1000 IU/70 uL)
Unit definition
One unit is defined as the amount of enzyme needed to cleave 3 ug of fusion protein in 1 hour to 85% completion at 30°C in a buffer containing 50 mM Tris-HCI, pH 8.0, 0.5mM EDTA, and 1 mM DTT.
Cleavage Conditions
A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4°C incubations are recommended. Reactions at 4°C will require longer incubation times and/or more rTEV.
Preparation and Storage
rTEV although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.
Related Product Information for TEV recombinant protein
Description: Recombinant TEV Protease (rTEV) is a site-specific protease purified from E Coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30 degree C; however, the enzyme can be used at temperatures as low as 4 degree C. The rTEV contains His tag.The rTEV is purified by proprietary chromatographic techniques.
Introduction: TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
Introduction: TEV protease is the common name for the 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the tobacco etch virus (TEV). Because its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins. TEV protease recognizes a linear epitope of the general form E-Xaa-Xaa-Y -Xaa-Q-(G/S), with cleavage occurring between Q and G or Q and S. The most commonly used sequence is ENLYFQG.
Product Categories/Family for TEV recombinant protein
NCBI and Uniprot Product Information
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Product Notes
The TEV (Catalog #AAA143255) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tobacco Etch Virus Protease, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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