Western Blot (WB)
((0. 01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PLA2G1B Polyclonal Antibody | anti-PLA2G1B antibody
Goat anti-PLA2G1B Antibody
Western Blot: Approx.17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx.18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 01ug/ml.
Western Blot (WB)
((0. 01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing PLA2G1B (RC216089) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB)
(HEK293 overexpressing PLA2G1B (RC216089) and probed (mock transfection in first lane), tested by Origene.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). The enzyme may be involved in several physiological processes including cell contraction, cell proliferation and pathological response. [provided by RefSeq, Aug 2013]
Uniprot Description
PLA2G1B: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.
Protein type: Secreted; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Phospholipase; EC 3.1.1.4; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; DNA replication
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: cell surface; extracellular region; extracellular space; secretory granule
Molecular Function: bile acid binding; calcium ion binding; calcium-dependent phospholipase A2 activity; phospholipase A2 activity; receptor binding
Biological Process: actin filament organization; activation of MAPK activity; activation of NF-kappaB transcription factor; activation of phospholipase A2; antibacterial humoral response; arachidonic acid secretion; cellular response to insulin stimulus; defense response to Gram-positive bacterium; fatty acid biosynthetic process; glucose transport; innate immune response in mucosa; interleukin-8 production; leukotriene biosynthetic process; multicellular organismal lipid catabolic process; neutrophil chemotaxis; neutrophil mediated immunity; phosphatidic acid biosynthetic process; phosphatidylcholine metabolic process; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of immune response; positive regulation of protein secretion; positive regulation of transcription from RNA polymerase II promoter; signal transduction
Research Articles on PLA2G1B
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Product Notes
The PLA2G1B pla2g1b (Catalog #AAA420861) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PLA2G1B Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PLA2G1B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx.17-18kDa band observed in Human Pancreas lysates (calculated MW of 16.4kDa according to NP_000919.1). In transfected HEK293 transiently expressing PLA2G1B a band of approx.18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0. 01ug/ml. Researchers should empirically determine the suitability of the PLA2G1B pla2g1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NKAHKNLDTK KYCQS. It is sometimes possible for the material contained within the vial of "PLA2G1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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