Goat Tenascin X ELISA Kit | TNX elisa kit

Goat Tenascin X ELISA Kit

Cat. Num. AAA741159

• Gene Names: TNXB; XB; TNX; XBS; EDS3; HXBL; TENX; TN-X; TNXB1; TNXB2; TNXBS
• Reactivity: Goat
• Synonyms: Tenascin X; Goat Tenascin X ELISA Kit; TNX elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This TNX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat TNX. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNX elisa kit

Principle of the assay: TNX ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TNX antibody and an TNX-HRP conjugate. The assay sample and buffer are incubated together with TNX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TNX concentration since TNX from samples and TNX-HRP conjugate compete for the anti-TNX antibody binding site. Since the number of sites is limited, as more sites are occupied by TNX from the sample, fewer sites are left to bind TNX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNX concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 20665034
• NCBI GeneID: 7148
• NCBI Accession #: NP_115859.2
• NCBI GenBank Nucleotide #: NM_032470.3
• UniProt Accession #: O95680; O95681; O95682; P22105; Q5JNX3; Q6IPK3; Q9NPK6; Q9NPK7; Q9NPK8; Q9UE34
• Molecular Weight: 46,087 Da
• NCBI Official Full Name: tenascin-X isoform 2
• NCBI Official Synonym Full Names: tenascin XB
• NCBI Official Symbol: TNXB
• NCBI Official Synonym Symbols: XB; TNX; XBS; EDS3; HXBL; TENX; TN-X; TNXB1; TNXB2; TNXBS
• NCBI Protein Information: tenascin-X; tenascin XB1; tenascin XB2; hexabrachion-like protein; growth-inhibiting protein 45
• UniProt Protein Name: Tenascin-X
• UniProt Gene Name: XB
• UniProt Entry Name: O95680_HUMAN

NCBI Description

This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TNXB: Appears to mediate interactions between cells and the extracellular matrix. Substrate-adhesion molecule that appears to inhibit cell migration. Accelerates collagen fibril formation. May play a role in supporting the growth of epithelial tumors. Defects in TNXB are the cause of tenascin-X deficiency (TNXD). TNXD leads to an Ehlers-Danlos-like syndrome characterized by hyperextensible skin, hypermobile joints, and tissue fragility. Tenascin-X-deficient patients, however, lack atrophic scars, a major diagnostic criteria for classic Ehlers- Danlos. Delayed wound healing, which is also common in classic EDS, is only present in a subset of patients. Belongs to the tenascin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; fibrillar collagen; intracellular

Molecular Function: integrin binding; heparin binding; collagen binding

Biological Process: elastic fiber assembly; cell-cell adhesion; collagen fibril organization; triacylglycerol metabolic process; collagen metabolic process; cell-matrix adhesion; fibril organization and biogenesis; regulation of JNK activity; actin cytoskeleton organization and biogenesis; cell adhesion; fatty acid metabolic process

Disease: Ehlers-danlos-like Syndrome Due To Tenascin-x Deficiency; Vesicoureteral Reflux 8