Trinucleotide Repeat Containing Protein 6A Recombinant Protein | TNRC6A recombinant protein

Recombinant Trinucleotide Repeat Containing Protein 6A (TNRC6A)

Cat. Num. AAA2033036

• Gene Names: TNRC6A; GW1; GW182; TNRC6; CAGH26
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Trinucleotide Repeat Containing Protein 6A; Recombinant Trinucleotide Repeat Containing Protein 6A (TNRC6A); CAGH26; GW1; GW182; TNRC6; CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen; Glycine-tryptophan protein of 182 kDa; TNRC6A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: T SAWSSIRASN YNVPLSSTAQ STSARNSDSK LTWSPGSVTN TSLAHELWKV PLPPKNITAP SRPPPGLTGQ KPPLSTWDNS PLRIGGGWGN SDARYTPGSS WGESSSGRIT NWLVLKNLTP QIDGSTLRTL CMQHGPLITF HLNLPHGNAL VRYSSKEEVV KAQKSLHMCV LGNTTILAEF ASEEEISRFF AQSQSLTPSP GWQSLGSSQS RLGSLDCSHS FSSRTDLNHW NGAGLSGTNCGDLHGTSLWG TPHYSTSLWG PPSSSDPRGI SSPSPINAFL SVDHLGGGGE SM
• Sequence Length: 1962

• Applicable Applications for TNRC6A recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 34.9kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Thr1670~Met1962
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 116805348
• NCBI GeneID: 27327
• NCBI Accession #: NP_055309.2
• NCBI GenBank Nucleotide #: NM_014494.2
• Molecular Weight: 205,151 Da
• NCBI Official Full Name: trinucleotide repeat-containing gene 6A protein
• NCBI Official Synonym Full Names: trinucleotide repeat containing 6A
• NCBI Official Symbol: TNRC6A
• NCBI Official Synonym Symbols: GW1; GW182; TNRC6; CAGH26
• NCBI Protein Information: trinucleotide repeat-containing gene 6A protein
• UniProt Protein Name: Trinucleotide repeat-containing gene 6A protein
• Protein Family: Trinucleotide repeat-containing gene 6A protein
• UniProt Gene Name: TNRC6A
• UniProt Synonym Gene Names: CAGH26; KIAA1460; TNRC6; Protein GW1
• UniProt Entry Name: TNR6A_HUMAN

NCBI Description

This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]

Uniprot Description

TNRC6A: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Belongs to the GW182 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cytosol; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: nucleotide binding; protein binding

Biological Process: cellular response to starvation; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; gene expression; innate immune response; miRNA-mediated gene silencing; miRNA-mediated gene silencing, negative regulation of translation; nerve growth factor receptor signaling pathway; Notch signaling pathway; phosphoinositide-mediated signaling; RNA-mediated gene silencing; RNA-mediated posttranscriptional gene silencing