Rat Troponin T, fast skeletal muscle ELISA Kit | Tnnt3 elisa kit

Rat Troponin T, fast skeletal muscle ELISA Kit

Cat. Num. AAA765209

• Gene Names: TNNT3; TNTF
• Reactivity: Rat
• Synonyms: Troponin T; fast skeletal muscle; Rat Troponin T; fast skeletal muscle ELISA Kit; Tnnt3/Troponin T; fast skeletal muscle/TnTf/Beta-TnTF/Fast skeletal muscle troponin T/fTnT; Tnnt3 elisa kit

• Reactivity: Rat
• Sequence Length: 250

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Tnnt3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Tnnt3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Tnnt3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Tnnt3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Tnnt3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 663071012
• NCBI GeneID: 7140
• NCBI Accession #: NP_001284575.1
• NCBI GenBank Nucleotide #: NM_001297646.1
• Molecular Weight: 29,735 Da
• NCBI Official Full Name: troponin T, fast skeletal muscle isoform 4
• NCBI Official Synonym Full Names: troponin T3, fast skeletal type
• NCBI Official Symbol: TNNT3
• NCBI Official Synonym Symbols: TNTF
• NCBI Protein Information: troponin T, fast skeletal muscle
• UniProt Protein Name: Troponin T, fast skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT3
• UniProt Synonym Gene Names: TnTf; fTnT
• UniProt Entry Name: TNNT3_HUMAN

NCBI Description

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]

Uniprot Description

TNNT3: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin T family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytosol; troponin complex

Molecular Function: actin binding; calcium-dependent ATPase activity; calcium-dependent protein binding; tropomyosin binding; troponin C binding; troponin I binding

Biological Process: muscle filament sliding; regulation of ATPase activity; regulation of striated muscle contraction; skeletal muscle contraction

Disease: Arthrogryposis, Distal, Type 2b