Troponin I, Cardiac Recombinant Protein | TNNT2 recombinant protein

Troponin I, Cardiac, Recombinant, Human (cTnI, CMH7, Familial Hypertrophic Cardiomyopathy 7, MGC116817, TNNC1, Troponin I Type 3 Cardiac, TNNI3)

Cat. Num. AAA636677

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
• Purity: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin:< 0.1ng/ug (IEU/ug).
• Synonyms: Troponin I; Cardiac; Recombinant; Human (cTnI; CMH7; Familial Hypertrophic Cardiomyopathy 7; MGC116817; TNNC1; Troponin I Type 3 Cardiac; TNNI3); TNNT2 recombinant protein

• Host: Recombinant, Human (E Coli).
• Purity/Purification: Highly Purified; 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin:< 0.1ng/ug (IEU/ug).
• Form/Format: Supplied as a liquid in 6M Urea, 50mM Tris, pH 8.0.

• Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis.
• Protein Content: Protein quantitation was carried out by:; 0.25-5.0mg/ml Bradford assay vs. BSA.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for TNNT2 recombinant protein

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits, the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI). Troponin I binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place, because of which myosin cannot bind to actin in relaxed muscle. When Calcium binds to the Troponin C it causes certain confomational changes which leads to dislocation of troponin I. The letter "I" is given due to its inhibitory character.

Recombinant Human cTnI is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 24,016D.

Product Categories/Family for TNNT2 recombinant protein

Molecular Biology; MB-Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 48255883
• NCBI GeneID: 7139
• NCBI Accession #: NP_001001432.1
• NCBI GenBank Nucleotide #: NM_001001432.2
• UniProt Accession #: P45379; O60214; Q99596; Q99597; Q9BUF6; Q9UM96; A2TDB9; A8K3K6
• Molecular Weight: 24kD
• NCBI Official Full Name: troponin T, cardiac muscle isoform 4
• NCBI Official Synonym Full Names: troponin T type 2 (cardiac)
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant)
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT
• UniProt Entry Name: TNNT2_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; actin binding; tropomyosin binding

Biological Process: atrial cardiac muscle morphogenesis; metabolic process; positive regulation of ATPase activity; regulation of muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2