TNNI1 recombinant protein

TNNI1, 1-187aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205292

• Gene Names: TNNI1; TNN1; SSTNI
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TNNI1; 1-187aa; Human; His tag; E Coli (Denatured); Troponin I slow skeletal muscle; Troponin I; slow skeletal muscle; DKFZp451O223; SSTNI; TNN1; TNNI1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP TLQTRGLSLS ALQDLCRELH AKVEVVDEER YDIEAKCLHN TREIKDLKLK VMDLRGKFKR PPLRRVRVSA DAMLRALLGS KHKVSMDLRA NLKSVKKEDT EKERPVEVGD WRKNVEAMSG MEGRKKMFDA AKSPTSQ
• Sequence Length: 187

• Applicable Applications for TNNI1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TNNI1 recombinant protein

Troponin I, slow skeletal muscle, also known as TNNI1, belongs to the troponin I family. TNNI1 is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Recombinant human TNNI1 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for TNNI1 recombinant protein

Cytoskeleton

References

Corin S.J., et al. (1994) J. Biol. Chem. 269:10651-10659; Wade R., et al. (1990) Genomics. 7:346-357;

NCBI and Uniprot Product Information

• NCBI GI #: 56682969
• NCBI GeneID: 7135
• NCBI Accession #: NP_003272
• NCBI GenBank Nucleotide #: NM_003281.3
• UniProt Accession #: P19237; Q659A5; Q6FGS7; Q6FGW1; Q6ICU2; Q86T57; Q96DT9; A6NEH3; A8MSJ0
• Molecular Weight: 23.8 kDa (207aa)
• NCBI Official Full Name: troponin I, slow skeletal muscle
• NCBI Official Synonym Full Names: troponin I1, slow skeletal type
• NCBI Official Symbol: TNNI1
• NCBI Official Synonym Symbols: TNN1; SSTNI
• NCBI Protein Information: troponin I, slow skeletal muscle
• UniProt Protein Name: Troponin I, slow skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI1

NCBI Description

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]

Uniprot Description

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.