TNNC1&TNNI3 recombinant protein

Recombinant Human TNNC1&TNNI3 protein, C-His Tag

Cat. Num. AAA157428

• Gene Names: TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13
• Purity: >90% as determined by SDS-PAGE
• Synonyms: TNNC1&TNNI3; Recombinant Human TNNC1&TNNI3 protein; C-His Tag; TNNC&TNNC1; TNNC1&TNNI3 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from 50 mM Tris-HCl pH7.5, 150 mM NaCl, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Source: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
• Construction: A DNA sequence encoding the human TNNI3 (Met1~Glu161 & Thr31~Arg111) was fused with the C-terminal His Tag
• Preparation and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for ~12 months from the date of receipt.; Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise

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Product Categories/Family for TNNC1&TNNI3 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 54042075
• NCBI GeneID: 7134
• UniProt Accession #: P63316; P19429
• Molecular Weight: 161
• NCBI Official Full Name: Troponin C, slow skeletal and cardiac muscles
• NCBI Official Synonym Full Names: troponin C type 1 (slow)
• NCBI Official Symbol: TNNC1
• NCBI Official Synonym Symbols: TNC; TN-C; TNNC; CMD1Z; CMH13
• NCBI Protein Information: troponin C, slow skeletal and cardiac muscles; troponin C1, slow; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C
• UniProt Protein Name: Troponin C, slow skeletal and cardiac muscles
• UniProt Gene Name: TNNC1
• UniProt Synonym Gene Names: TNNC; TN-C
• UniProt Entry Name: TNNC1_HUMAN

NCBI Description

Troponin is a central regulatory protein of striated muscle contraction, and together with tropomyosin, is located on the actin filament. Troponin consists of 3 subunits: TnI, which is the inhibitor of actomyosin ATPase; TnT, which contains the binding site for tropomyosin; and TnC, the protein encoded by this gene. The binding of calcium to TnC abolishes the inhibitory action of TnI, thus allowing the interaction of actin with myosin, the hydrolysis of ATP, and the generation of tension. Mutations in this gene are associated with cardiomyopathy dilated type 1Z. [provided by RefSeq, Oct 2008]

Uniprot Description

TNNC1: Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Defects in TNNC1 are the cause of cardiomyopathy dilated type 1Z (CMD1Z). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Defects in TNNC1 are the cause of familial hypertrophic cardiomyopathy type 13 (CMH13). A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Belongs to the troponin C family.

Protein type: Calcium-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; troponin complex; mitochondrion; cytosol; actin cytoskeleton

Molecular Function: troponin T binding; actin filament binding; protein binding; protein homodimerization activity; troponin I binding; calcium ion binding; calcium-dependent protein binding

Biological Process: response to metal ion; regulation of ATPase activity; regulation of muscle contraction; diaphragm contraction; ventricular cardiac muscle morphogenesis; regulation of muscle filament sliding speed; cardiac muscle contraction; muscle filament sliding

Disease: Cardiomyopathy, Dilated, 1z; Cardiomyopathy, Familial Hypertrophic, 13