Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2) Recombinant Protein | TNIP2 recombinant protein

Recombinant Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2)

Cat. Num. AAA2010998

• Gene Names: Tnip2; ABIN-2; AI428870; 1810020H16Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2); Recombinant Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2); TNIP2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- A KCLDERQCAQ GDAGEKSPAE LEQTSSDASG QSVIKKLQEE NRLLKQKVTH VEDLNAKWQR YDASRDEYVK GLHAQLKRRQ VPLEPELMKK EISRLNRQLE EKISDCAEAN QELTAMRMSR DTALERVQML EQQILAYKDD FKSERADRER AHSRIQELEE KIMSLMYQVS QRQDSREPGP CRIHTGNKTA KYLEMDALEH VTPGGWRPES RSQQMEPSAE GGHVCTAQRG QGDLQCPHCL RCF
• Sequence Length: 430

• Applicable Applications for TNIP2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ala170~Phe413 (Accession # Q99JG7) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 20532344
• NCBI GeneID: 231130
• NCBI Accession #: NP_620703.1
• NCBI GenBank Nucleotide #: NM_139064.2
• UniProt Accession #: Q99JG7; Q3TVF3
• Molecular Weight: 31.9kDa
• NCBI Official Full Name: TNFAIP3-interacting protein 2
• NCBI Official Synonym Full Names: TNFAIP3 interacting protein 2
• NCBI Official Symbol: Tnip2
• NCBI Official Synonym Symbols: ABIN-2; AI428870; 1810020H16Rik
• NCBI Protein Information: TNFAIP3-interacting protein 2; A20 binding inhibitor of NF-kappaB activation-2; A20-binding inhibitor of NF-kappa-B activation 2
• UniProt Protein Name: TNFAIP3-interacting protein 2
• Protein Family: TNFAIP3-interacting protein
• UniProt Gene Name: Tnip2
• UniProt Synonym Gene Names: Abin2; ABIN-2
• UniProt Entry Name: TNIP2_MOUSE

Uniprot Description

TNIP2: Inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. Forms a ternary complex with NFKB1 and MAP3K8 but appears to function upstream of MAP3K8 in the TLR4 signaling pathway that regulates MAP3K8 activation. Involved in activation of the MEK/ERK signaling pathway during innate immune response; this function seems to be stimulus- and cell type specific. Required for stability of MAP3K8. Involved in regulation of apoptosis in endothelial cells; promotes TEK agonist-stimulated endothelial survival. May act as transcriptional coactivator when translocated to the nucleus. Enhances CHUK-mediated NF-kappa-B activation involving NF-kappa-B p50-p65 and p50-c-Rel complexes. 2 isoforms of the human protein are produced by alternative splicing.

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; polyubiquitin binding; protein kinase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; positive regulation of B cell activation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; toll-like receptor 3 signaling pathway; inflammatory response; positive regulation of macrophage activation; toll-like receptor 2 signaling pathway