APRIL/TNFSF13 recombinant protein

Recombinant Human APRIL/TNFSF13 Protein (N-DYKDDDDK-His)

Cat. Num. AAA2557393

• Gene Names: TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: APRIL/TNFSF13; Recombinant Human APRIL/TNFSF13 Protein (N-DYKDDDDK-His); Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1; TRDL-1; CD256; TNFSF13; TALL2; APRIL/TNFSF13 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Lys112-Leu250
• Sequence Length: 222

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: N-DYKDDDDK-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for APRIL/TNFSF13 recombinant protein

Protein Construction: Recombinant Human APRIL is produced by our mammalian expression system and the target gene encoding Lys112-Leu250 is expressed with a His, DYKDDDDK tag at the N-terminus.

Background: APRIL (a proliferation-inducingligand), also known as TNFSF13, TALL2, TRDL1, and CD256, is a member of the TNF ligand superfamily. It is synthesized as a32 kDa proprotein which is cleaved by furin in the Golgi to release the active 17 kDa soluble molecule. Secreted human APRIL, which consists almost entirely ofa single TNF homology domain, shares 85% amino acid sequence identity with mouse and rat APRIL. Both APRIL and its close relative BAFF bind and signalthrough the TNF superfamily receptors TACI and BCMA, while BAFF additionally functions through BAFF R. APRIL binds to heparan sulfateproteoglycans (HSPGs) independently of its binding to TACI and BCMA. APRIL can form bioactive heterotrimers withBAFF, and these circulate in the serum of patients with rheumatic immune disorders. APRIL enhances the proliferation and survival of plasma cells andalso promotes T cell-dependenthumoral responses. APRIL levels are elevated inthe serum during coronary artery disease, and it is also elevated in many cancers primarily due to expression by tumor-infiltratingneutrophils.

NCBI and Uniprot Product Information

• NCBI GI #: 310750387
• NCBI GeneID: 8741
• NCBI Accession #: NP_001185552.1
• NCBI GenBank Nucleotide #: NP_001185552.1
• UniProt Accession #: O75888
• Molecular Weight: Molecular Mass: 50kDa; Actual Protein Molecular Mass: 60kDa
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13 isoform zeta
• NCBI Official Synonym Full Names: TNF superfamily member 13
• NCBI Official Symbol: TNFSF13
• NCBI Official Synonym Symbols: APRIL; CD256; TALL2; ZTNF2; TALL-2; TNLG7B; TRDL-1; UNQ383/PRO715
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13
• UniProt Gene Name: TNFSF13
• UniProt Synonym Gene Names: APRIL; TALL2; ZTNF2; APRIL; TALL-2; TRDL-1
• UniProt Entry Name: TNF13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol; external side of plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; gene expression; signal transduction; positive regulation of isotype switching to IgA isotypes