Human tumor necrosis factor (ligand) superfamily, member 13 ELISA Kit | TNFSF13 elisa kit

Human a proliferation inducing ligand, APRIL ELISA Kit

Cat. Num. AAA705404

• Gene Names: TNFSF13; APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
• Reactivity: Human
• Synonyms: tumor necrosis factor (ligand) superfamily; member 13; Human a proliferation inducing ligand; APRIL ELISA Kit; Human a proliferation inducing ligand (APRIL) ELISA Kit; APRIL; CD256; TALL2; TRDL-1; UNQ383/PRO715; ligand; TNF- and APOL-related leukocyte expressed ligand 2; a proliferation inducing ligand; tumor necrosis factor (ligand) superfamily member 13 transcript; TNFSF13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human APRIL. No significant cross-reactivity or interference between human APRIL and analogues was observed.
• Sequence Length: 222

• Samples: Serum, plasma, urine
• Assay Type: Sandwich
• Detection Range: 0.78 ng/ml -50 ng/ml.
• Sensitivity: The minimum detectable dose of human APRIL is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNFSF13 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APRIL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APRIL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APRIL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APRIL bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 310750387
• NCBI GeneID: 8741
• NCBI Accession #: NP_001185552.1
• NCBI GenBank Nucleotide #: NM_001198623.1
• UniProt Accession #: O75888; Q541E1; Q5U0G8; Q96HV6; Q9P1M8; Q9P1M9; A8MYD5; B4DVT2
• Molecular Weight: 27,433 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 13 isoform zeta
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 13
• NCBI Official Symbol: TNFSF13
• NCBI Official Synonym Symbols: APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 13
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF13
• UniProt Synonym Gene Names: APRIL; TALL2; ZTNF2; UNQ383/PRO715; APRIL; TALL-2; TRDL-1
• UniProt Entry Name: TNF13_HUMAN

NCBI Description

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

Function: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.1 PublicationManual assertion based on experiment in:Ref.12