TNF Ligand Superfamily Recombinant Protein | TNFSF12 recombinant protein

Recombinant Human TNF Ligand Superfamily Member 12

Cat. Num. AAA143906

• Gene Names: TNFSF12; APO3L; DR3LG; TWEAK
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: TNF Ligand Superfamily; Recombinant Human TNF Ligand Superfamily Member 12; TNFSF12 Human; TNF Ligand Superfamily Member 12 Human Recombinant; TWEAK; TNF-related weak inducer of apoptosis; TNFSF12; DR3LG; Apo3-Ligand; APO3L; TNFRSF12A; Tumor necrosis factor ligand superfamily member 12; MGC20669; MGC129581; TNFSF12 recombinant protein

• Host: HEK293
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: MHHHHHHRSA PKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH
• Sequence Length: 249

• Solubility: Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized TWEAK Human recombinant at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for TNFSF12 recombinant protein

Description: The TWEAK Human Recombinant is manufactured with N-terminal fusion of 13 amino acid DYKDDDDK tag. The TNFSF12 DYKDDDDK-Tagged Fusion Protein has a Mw of 18.7kDa containing a total of 169 amino acid residues.

Introduction: TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-?. TNFSF12 promotes proliferation and migration of endothelial cells.

Product Categories/Family for TNFSF12 recombinant protein

Cytokines and Growth Factors; Cytokines; Tumor Necrosis Factor

NCBI and Uniprot Product Information

• NCBI GI #: 4507597
• NCBI GeneID: 8742
• NCBI Accession #: NP_003800.1
• NCBI GenBank Nucleotide #: NM_003809.2
• UniProt Accession #: O43508; Q8IZK7; Q8WUZ7
• Molecular Weight: 36,589 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 12 proprotein
• NCBI Official Synonym Full Names: tumor necrosis factor (ligand) superfamily, member 12
• NCBI Official Symbol: TNFSF12
• NCBI Official Synonym Symbols: APO3L; DR3LG; TWEAK
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 12; APO3 ligand; APO3/DR3 ligand; TNF-related WEAK inducer of apoptosis
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 12
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: TNFSF12
• UniProt Synonym Gene Names: APO3L; DR3LG; TWEAK
• UniProt Entry Name: TNF12_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

Uniprot Description

TNFSF12: Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Homotrimer (Potential). Interacts with the angiogenic factor AGGF1/VG5Q. Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain. Belongs to the tumor necrosis factor family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Apoptosis; Cytokine

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: extracellular space; perinuclear region of cytoplasm; integral to plasma membrane

Molecular Function: protein binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: positive regulation of angiogenesis; apoptosis; positive regulation of protein catabolic process; endothelial cell migration; immune response; positive regulation of endothelial cell proliferation; angiogenesis; cell differentiation; signal transduction