TNFRSF1B/CD120b/TNFR2 Recombinant Protein | TNFRSF1B recombinant protein

Recombinant Human TNFRSF1B/CD120b/TNFR2 Protein (C-mFc)

Cat. Num. AAA2557093

• Gene Names: TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• Applications: SDS-Page
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: TNFRSF1B/CD120b/TNFR2; Recombinant Human TNFRSF1B/CD120b/TNFR2 Protein (C-mFc); Tumor necrosis factor receptor superfamily member 1B; TNFRSF1B; Tumor necrosis factor receptor 2; TNF-R2; TNF-RII; Tumor necrosis factor receptor type II; p75; p80 TNF-alpha receptor; CD120b; Tumor Necrosis Factor Receptor II; TNFRSF1B recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
• Sequence: Pro24-Thr206
• Sequence Length: 461

• Applicable Applications for TNFRSF1B recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: C-mFc
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom.Do not vortex .; It is recommended that the sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is mesured by UV-Vis.
• Preparation and Storage: Storage: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.; Reconstituted protein solution can be stored at 4-7°C for 2-7days.; Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for TNFRSF1B recombinant protein

Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) is a member of the tumor necrosis factor receptor superfamily. Human TNF RII contains four cysteinerich repeats in its ECD, which shares 58% and 56% amino acid sequence identity with the mouse and rat orthologs, respectively. TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, thymocytes, and mesenchymal stem cells. TNF RII binds to the membranebound forms of TNF-Alpha and Lymphotoxin?/TNF-Beta soluble TNF is thought to signal predominately through TNF RI. Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors.

NCBI and Uniprot Product Information

• NCBI GI #: 4507577
• NCBI GeneID: 7133
• NCBI Accession #: NP_001057.1
• NCBI GenBank Nucleotide #: NP_001057.1
• UniProt Accession #: P20333
• Molecular Weight: Molecular Mass: 46.44kDa; Actual Protein Molecular Mass: 60kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1B
• NCBI Official Synonym Full Names: TNF receptor superfamily member 1B
• NCBI Official Symbol: TNFRSF1B
• NCBI Official Synonym Symbols: p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1B
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1B
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF1B
• UniProt Synonym Gene Names: TNFBR; TNFR2; TNF-R2; TNF-RII; TNFR-II
• UniProt Entry Name: TNR1B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-R2: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. Binds to TRAF2. Interacts with BMX. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cell soma; perinuclear region of cytoplasm; extracellular region; integral to membrane; plasma membrane; nucleus; lipid raft

Molecular Function: protein binding; tumor necrosis factor receptor activity; ubiquitin protein ligase binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; tumor necrosis factor-mediated signaling pathway; negative regulation of inflammatory response; immune response; RNA destabilization; inflammatory response; positive regulation of membrane protein ectodomain proteolysis; aging