GITR/TNFRSF18 recombinant protein

Recombinant Human GITR/TNFRSF18 Protein (C-mFc)

Cat. Num. AAA2557277

• Gene Names: TNFRSF18; AITR; GITR; CD357; GITR-D; ENERGEN
• Purity: >90% as determined by reducing SDS-PAGE.
• Synonyms: GITR/TNFRSF18; Recombinant Human GITR/TNFRSF18 Protein (C-mFc); Tumor necrosis factor receptor superfamily member 18; TNFRSF18; Glucocorticoid-induced TNFR-related protein; CD357; AITR; GITR; GITR/TNFRSF18 recombinant protein

• Host: Human Cells
• Purity/Purification: >90% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH7.4.
• Sequence: Gln26-Glu161 (Thr45Ala)
• Sequence Length: 241

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-mFc
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for GITR/TNFRSF18 recombinant protein

Protein Construction: Recombinant human GITR is produced by our mammalian expression system and the target gene encoding Gln26-Glu161(Thr45Ala) is expressed with a mFc tag at the C-terminus.

Background: Tumor necrosis factor receptor superfamily member 18(Gitr) contains 3 TNFR-Cys repeats and it have four isforms.IsformA?isformB and isformC is single-pass type I membrane protein and isformD is a secreted protein. The protein is the receptor for TNFSF18.It seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. It mediated NF-kappa-B activation via the TRAF2/NIK pathway.It binds to TRAF1, TRAF2, and TRAF3, but not TRAF5 and TRAF6 and binds through its C-terminus to SIVA1/SIVA.It preferentially expressed in activated T lymphocytes and up-regulated in peripherical mononuclear cells after antigen stimulation/lymphocyte activation.

NCBI and Uniprot Product Information

• NCBI GI #: 4558503
• NCBI GeneID: 8784
• UniProt Accession #: Q9Y5U5
• Molecular Weight: Molecular Mass: 41.1kDa; Actual Protein Molecular Mass: 40-55kDa
• NCBI Official Full Name: glucocorticoid-induced TNFR-related protein
• NCBI Official Synonym Full Names: TNF receptor superfamily member 18
• NCBI Official Symbol: TNFRSF18
• NCBI Official Synonym Symbols: AITR; GITR; CD357; GITR-D; ENERGEN
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 18
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 18
• UniProt Gene Name: TNFRSF18
• UniProt Synonym Gene Names: AITR; GITR
• UniProt Entry Name: TNR18_HUMAN

NCBI Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFRSF18: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: tumor necrosis factor receptor activity

Biological Process: tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction; negative regulation of apoptosis