Mouse anti-Human, Mouse SQSTM1 Monoclonal Antibody | anti-SQSTM1 antibody

SQSTM1 (Sequestosome-1, EBI3-associated Protein of 60kD, EBIAP, p60, ORCA, OSIL, Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD, Ubiquitin-binding Protein p62) (MaxLight 650)

Cat. Num. AAA6224859

• Gene Names: SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SQSTM1; Monoclonal Antibody; SQSTM1 (Sequestosome-1; EBI3-associated Protein of 60kD; EBIAP; p60; ORCA; OSIL; Phosphotyrosine-independent Ligand for the Lck SH2 Domain of 62kD; Ubiquitin-binding Protein p62) (MaxLight 650); A170; p62; p62B; PDB3; ZIP3; anti-SQSTM1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2C11
• Specificity: Recognizes human SQSTM1. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-SQSTM1 antibody: FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant protein corresponding to aa1-440 from human SQSTM1 with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL
• Conjugate: MaxLight650
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against immunogen using 133821 (74.51kD).)

Testing Data

(Detection limit is ~0.03ng/ml using 133821 as a capture antibody.)

Immunofluorescence (IF)

(Immunofluorescence to SQSTM1 on HeLa cell using 133821 (10ug/ml).)

Related Product Information for anti-SQSTM1 antibody

MaxLight650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor647, DyLight649, Cy5 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Product Categories/Family for anti-SQSTM1 antibody

Antibodies; Ubiquitin

References

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NCBI and Uniprot Product Information

• NCBI GI #: 13111936
• NCBI GeneID: 8878
• Molecular Weight: 38,629 Da
• NCBI Official Full Name: Homo sapiens sequestosome 1, mRNA
• NCBI Official Synonym Full Names: sequestosome 1
• NCBI Official Symbol: SQSTM1
• NCBI Official Synonym Symbols: p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
• NCBI Protein Information: sequestosome-1
• Protein Family: Sequestosome

NCBI Description

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]