Human Tumor necrosis factor receptor superfamily member 13C ELISA Kit | TNFRSF13C/BAFFR elisa kit

Human Tumor necrosis factor receptor superfamily member 13C ELISA Kit

Cat. Num. AAA761541

• Gene Names: TNFRSF13C; BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
• Reactivity: Human
• Synonyms: Tumor necrosis factor receptor superfamily member 13C; Human Tumor necrosis factor receptor superfamily member 13C ELISA Kit; BAFFR/TNFRSF13C/AFF-R/BROMIX/CD268/CVID4/Prolixin/Tumor Necrosis Factor Receptor Superfamily Member 13C/B Cell Activating Factor Receptor; TNFRSF13C/BAFFR elisa kit

• Reactivity: Human
• Sequence Length: 184

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for TNFRSF13C/BAFFR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human BAFFRantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human BAFFR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human BAFFR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman BAFFRcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16445027
• NCBI GeneID: 115650
• NCBI Accession #: NP_443177.1
• NCBI GenBank Nucleotide #: NM_052945.3
• Molecular Weight: 18,935 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 13C
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily member 13C
• NCBI Official Symbol: TNFRSF13C
• NCBI Official Synonym Symbols: BAFFR; CD268; CVID4; BAFF-R; BROMIX; prolixin
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 13C
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 13C
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF13C
• UniProt Synonym Gene Names: BAFFR; BR3; BAFF-R
• UniProt Entry Name: TR13C_HUMAN

NCBI Description

B cell-activating factor (BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. Overexpression of Baff in mice results in mature B-cell hyperplasia and symptoms of systemic lupus erythematosus (SLE). Also, some SLE patients have increased levels of BAFF in serum. Therefore, it has been proposed that abnormally high levels of BAFF may contribute to the pathogenesis of autoimmune diseases by enhancing the survival of autoreactive B cells. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular cysteine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival. [provided by RefSeq, Jul 2008]

Uniprot Description

BAFF-R: B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response. Defects in TNFRSF13C are the cause of immunodeficiency common variable type 4 (CVID4); also called antibody deficiency due to BAFFR defect. CVID4 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Membrane protein, integral; Cell surface; Receptor, misc.

Chromosomal Location of Human Ortholog: 22q13.1-q13.31

Cellular Component: external side of plasma membrane; integral to membrane; plasma membrane

Biological Process: adaptive immune response; B cell costimulation; B cell homeostasis; positive regulation of B cell proliferation; positive regulation of germinal center formation; positive regulation of interferon-gamma biosynthetic process; positive regulation of T cell proliferation; T cell costimulation; tumor necrosis factor-mediated signaling pathway

Disease: Immunodeficiency, Common Variable, 2; Immunodeficiency, Common Variable, 4