TRAIL R1 / CD261 / TNFRSF10A recombinant protein

Recombinant Human TRAIL R1 / CD261 / TNFRSF10A Protein (Fc tag)

Cat. Num. AAA2546279

• Gene Names: TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: TRAIL R1 / CD261 / TNFRSF10A; Recombinant Human TRAIL R1 / CD261 / TNFRSF10A Protein (Fc tag); TNFRSF10A; APO2; CD261; DR4; MGC9365; TRAILR-1; TRAILR1; TRAIL R1 / CD261 / TNFRSF10A recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 468

• Application Notes: The recombinant human TNFRSF10A/Fc is a disulfide-linked homodimer. The reduced monomer consists of 372 amino acids and has a predicted molecular mass of 41.3 kDa. As a result of glycosylation, rh TNFRSF10A/Fc monomer migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 109
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TRAIL R1 / CD261 / TNFRSF10A recombinant protein

Background: Tumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell type s.

Description: A DNA sequence encoding the human TNFRSF10A (NP_003835.2) extracellular domain (Met 1-Asn 239) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 21361086
• NCBI GeneID: 8797
• NCBI Accession #: NP_003835.2
• NCBI GenBank Nucleotide #: NM_003844.2
• Molecular Weight: 50,089 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily, member 10a
• NCBI Official Synonym Full Names: TNF receptor superfamily member 10a
• NCBI Official Symbol: TNFRSF10A
• NCBI Official Synonym Symbols: DR4; APO2; CD261; TRAILR1; TRAILR-1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10A
• UniProt Gene Name: TNFRSF10A
• UniProt Synonym Gene Names: APO2; DR4; TRAILR1; TRAIL receptor 1; TRAIL-R1

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIL-R1: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: cell surface; integral to membrane; integral to plasma membrane; plasma membrane

Molecular Function: death receptor activity; protease binding; protein binding; transcription factor binding; tumor necrosis factor receptor activity

Biological Process: caspase activation; cell surface receptor linked signal transduction; immune response; inflammatory response; multicellular organismal development; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide; signal transduction