TNF ALPHA recombinant protein
RECOMBINANT BOVINE TNF ALPHA
Gene Names
TNF; TNFa; TNF-a; TNF-alpha
Applications
ELISA, Functional Assay
Purity
>95% by SDS PAGE analysis
Synonyms
TNF ALPHA; RECOMBINANT BOVINE TNF ALPHA; TNF ALPHA recombinant protein
Purity/Purification
>95% by SDS PAGE analysis
Form/Format
Purified Recombinant Protein- lyophilised
Sequence Length
234
Applicable Applications for TNF ALPHA recombinant protein
ELISA (EIA), Functional Assays (FN)
Reconstitution
Reconstitute with 0.5 ml distilled water. Further dilutions should be made in a buffer containing carrier protein.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Preparation
Preparation
Endotoxin Level
less than 0.7 EU/ug
Buffer Solution
Buffer Solution
Target Species
Bovine
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -70 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Related Product Information for TNF ALPHA recombinant protein
Bovine Tumour Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages, monocytes, T and B lymphocytes. TNF alpha is an important mediator of the bovine inflammatory response. MBS232226 has an ED50 in the range of 10-25 ng/ml, determined by measuring the cytolytic effect of recombinant bovine TNF alpha on murine WEHI-13VAR cells in the presence of Actinomycin D.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Protein Molecular Weight: 17.4kD
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor<
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
TNFa; TNF-a; TNF-alpha
NCBI Protein Information
tumor necrosis factor; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_BOVIN
Uniprot Description
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Research Articles on TNF ALPHA
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Product Notes
The TNF ALPHA tnf (Catalog #AAA232226) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNF ALPHA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Functional Assays (FN). Researchers should empirically determine the suitability of the TNF ALPHA tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF ALPHA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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