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Enterokinase Recombinant Protein | ENTK recombinant protein

Recombinant Enterokinase (ENTK)

Applications
SDS-Page, Western Blot
Purity
>97%
Synonyms
Enterokinase; Recombinant Enterokinase (ENTK); PRSS7; TMPRSS15; Protease; Serine 7; Proenterokinase; Enteropeptidase; Transmembrane protease serine 15; ENTK recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
>97%
Form/Format
Freeze-dried powder
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence Positions
Gln902~His1069
Sequence Length
1069
Applicable Applications for ENTK recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Mouse
Tag
N-terminal His Tag
Subcellular Location
Secreted
Isoelectric Point
5.3
Reconstitution
Reconstitute in PBS or others.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Product Categories/Family for ENTK recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 22.6kDa
Accurate Molecular Mass: 25kDa
NCBI Official Full Name
enteropeptidase isoform 1
UniProt Protein Name
Enteropeptidase
UniProt Gene Name
Tmprss15
UniProt Synonym Gene Names
Entk; Prss7
UniProt Entry Name
ENTK_MOUSE

Uniprot Description

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: Protease; EC 3.4.21.9; Membrane protein, integral

Cellular Component: integral to membrane; membrane

Molecular Function: hydrolase activity; peptidase activity; scavenger receptor activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: proteolysis

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Product Notes

The ENTK tmprss15 (Catalog #AAA2123750) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Gln902~His1069. AAA Biotech's Enterokinase can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the ENTK tmprss15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enterokinase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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