Human Toll Like Receptor 6 (TLR6) ELISA Kit | TLR6 elisa kit

Human Toll Like Receptor 6 (TLR6) ELISA Kit

Cat. Num. AAA2018971

• Gene Names: TLR6; CD286
• Reactivity: Human
• Synonyms: Toll Like Receptor 6 (TLR6); Human Toll Like Receptor 6 (TLR6) ELISA Kit; CD286; TLR6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TLR6. No significant cross-reactivity or interference between TLR6 and analogues was observed.
• Sequence Length: 796

• Samples: Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.127ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TLR6 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TLR6 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR6 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TLR6 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TLR6. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TLR6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TLR6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TLR6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TLR6 elisa kit

Tumor immunity; Infection immunity; Immune molecule; Pulmonology

NCBI and Uniprot Product Information

• NCBI GI #: 20143971
• NCBI GeneID: 10333
• NCBI Accession #: NP_006059.2
• NCBI GenBank Nucleotide #: NM_006068.4
• UniProt Accession #: Q9Y2C9; Q2NKL3; B3Y640; B6CH35; B6RFS4; B6RFS5
• Molecular Weight: 55,828 Da
• NCBI Official Full Name: toll-like receptor 6
• NCBI Official Synonym Full Names: toll-like receptor 6
• NCBI Official Symbol: TLR6
• NCBI Official Synonym Symbols: CD286
• NCBI Protein Information: toll-like receptor 6
• UniProt Protein Name: Toll-like receptor 6
• UniProt Gene Name: TLR6
• UniProt Entry Name: TLR6_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor functionally interacts with toll-like receptor 2 to mediate cellular response to bacterial lipoproteins. A Ser249Pro polymorphism in the extracellular domain of the encoded protein may be associated with an increased of asthma is some populations.[provided by RefSeq, Jan 2011]

Uniprot Description

TLR6: Participates in the innate immune response to Gram- positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. Belongs to the Toll-like receptor family.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; T-helper 1 type immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; detection of diacylated bacterial lipoprotein; positive regulation of interleukin-6 biosynthetic process; activation of NF-kappaB-inducing kinase; signal transduction; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 6 signaling pathway; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; immune response; inflammatory response; toll-like receptor 4 signaling pathway