TLR3 recombinant protein

TLR3, 23-704aa, Human, His-tag, Baculovirus

Cat. Num. AAA206810

• Gene Names: TLR3; CD283; IIAE2
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: TLR3; 23-704aa; Human; His-tag; Baculovirus; Toll-like receptor 3; CD283; IIAE2; TLR3 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: SSTTKCTVSH EVADCSHLKL TQVPDDLPTN ITVLNLTHNQ LRRLPAANFT RYSQLTSLDV GFNTISKLEP ELCQKLPMLK VLNLQHNELS QLSDKTFAFC TNLTELHLMS NSIQKIKNNP FVKQKNLITL DLSHNGLSST KLGTQVQLEN LQELLLSNNK IQALKSEELD IFANSSLKKL ELSSNQIKEF SPGCFHAIGR LFGLFLNNVQ LGPSLTEKLC LELANTSIRN LSLSNSQLST TSNTTFLGLK WTNLTMLDLS YNNLNVVGND SFAWLPQLEY FFLEYNNIQH LFSHSLHGLF NVRYLNLKRS FTKQSISLAS LPKIDDFSFQ WLKCLEHLNM EDNDIPGIKS NMFTGLINLK YLSLSNSFTS LRTLTNETFV SLAHSPLHIL NLTKNKISKI ESDAFSWLGH LEVLDLGLNE IGQELTGQEW RGLENIFEIY LSYNKYLQLT RNSFALVPSL QRLMLRRVAL KNVDSSPSPF QPLRNLTILD LSNNNIANIN DDMLEGLEKL EILDLQHNNL ARLWKHANPG GPIYFLKGLS HLHILNLESN GFDEIPVEVF KDLFELKIID LGLNNLNTLP ASVFNNQVSL KSLNLQKNLI TSVEKKVFGP AFRNLTELDM RFNPFDCTCE SIAWFVNWIN ETHTNIPELS SHYLCNTPPH YHGFPVRLFD TSSCKDSAPF ELLEHHHHHH
• Sequence Length: 904

• Applicable Applications for TLR3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1ug of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TLR3 recombinant protein

TLR3, also known as toll-like receptor 3, is a member of the toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. It is innate immune receptors for sensing microbial molecules and damage-associated molecular patterns released from host cells. It recognizes dsRNA and activation of the receptor induces the activation of NF-kappaB and the production of type I interferons (IFNs). Its signaling activation is associated with ischemic preconditioning-induced protection against brain ischemia and attenuation of reactive astrogliosis. It is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. Recombinant human TLR3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for TLR3 recombinant protein

Immunology

References

Alexopoulou L., et al. (2001) Nature. 413:732-738. Cole JE., et al. (2011) Proc Natl Acad Sci U S A. 108:2372-2377.

NCBI and Uniprot Product Information

• NCBI GI #: 4507531
• NCBI GeneID: 7098
• NCBI Accession #: NP_003256
• NCBI GenBank Nucleotide #: NM_003265.2
• UniProt Accession #: O15455; Q4VAL2; Q504W0; B2RAI7; B7Z7K0; E6Y0F0; E6Y0F1; E9PGH4
• Molecular Weight: 78.5kDa (690aa) 70-100kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: toll-like receptor 3
• NCBI Official Synonym Full Names: toll like receptor 3
• NCBI Official Symbol: TLR3
• NCBI Official Synonym Symbols: CD283; IIAE2
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR3

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response.