TK2 recombinant protein

TK2, 34-265aa, Human, His tag, E Coli

Cat. Num. AAA204339

• Gene Names: TK2; MTTK; PEOB3; SCA31; MTDPS2
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: TK2; 34-265aa; Human; His tag; E Coli; thymidine kinase 2 mitochondrial; thymidine kinase 2; mitochondrial; MTDPS2; MTTK; TK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 30% glycerol, 2mM DTT, 200mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVQRRA WPPDKEQEKE KKSVICVEGN IASGKTTCLE FFSNATDVEV LTEPVSKWRN VRGHNPLGLM YHDASRWGLT LQTYVQLTML DRHTRPQVSS VRLMERSIHS ARYIFVENLY RSGKMPEVDY VVLSEWFDWI LRNMDVSVDL IVYLRTNPET CYQRLKKRCR EEEKVIPLEY LEAIHHLHEE WLIKGSLFPM AAPVLVIEAD HHMERMLELF EQNRDRILTP ENRKHCP
• Sequence Length: 265

• Applicable Applications for TK2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for TK2 recombinant protein

TK2, also known as thymidine kinase 2 mitochondrial, belongs to the DCK-DGK family. Thymidine kinase is an enzyme, a phosphotransferase (a kinase): 2-deoxythymidine kinase, ATP-thymidine 5-phosphotransferase, It can be found in most living cells. It is present in two forms in mammalian cells, TK1 and TK2. Certain viruses also have genetic information for expression of viral thymidine kinases. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2). A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. Recombinant human TK2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for TK2 recombinant protein

Enzymes & Proteases

References

Saada A., et al. (2001) Nat. Genet. 29:342-344; Kit S. (1985). Thymidine kinase. Microbiol. Sci. 2 (12): 369-75.

NCBI and Uniprot Product Information

• NCBI GI #: 290656936
• NCBI GeneID: 7084
• NCBI Accession #: NP_004605
• NCBI GenBank Nucleotide #: NM_004614.4
• UniProt Accession #: O00142; O15238; B4DGJ7; B4DZK7; B7ZAB1; E5KNQ5; E9PH08
• Molecular Weight: 30.2kDa (257aa), confirmed by MALDI-TOF
• NCBI Official Full Name: thymidine kinase 2, mitochondrial isoform 1
• NCBI Official Synonym Full Names: thymidine kinase 2, mitochondrial
• NCBI Official Symbol: TK2
• NCBI Official Synonym Symbols: MTTK; PEOB3; SCA31; MTDPS2
• NCBI Protein Information: thymidine kinase 2, mitochondrial; thymidine kinase 2
• UniProt Protein Name: Thymidine kinase 2, mitochondrial
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK2

NCBI Description

This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria. [provided by RefSeq, Dec 2012]

Uniprot Description

Phosphorylates thymidine, deoxycytidine, and deoxyuridine in the mitochondrial matrix. In non-replicating cells, where cytosolic dNTP synthesis is down-regulated, mtDNA synthesis depends solely on TK2 and DGUOK. Widely used as target of antiviral and chemotherapeutic agents.