Rabbit anti-Human Thymidine Kinase 2 Polyclonal Antibody | anti-TK2 antibody

Thymidine Kinase 2 Antibody (C-term)

Cat. Num. AAA9232348

• Gene Names: TK2; MTTK; SCA31; MTDPS2
• Reactivity: Human
• Applications: Western Blot
• Synonyms: Thymidine Kinase 2; Polyclonal Antibody; Thymidine Kinase 2 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); Thymidine kinase 2; mitochondrial; Mt-TK; TK2; anti-TK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB5435
• Specificity: This Thymidine Kinase 2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 233-263 amino acids from the C-terminal region of human Thymidine Kinase 2.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Applicable Applications for anti-TK2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Antigen Source: Human
• Antigen Region: 233-263
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-TK2 Pab is used in Western blot to detect TK2 in mouse muscle tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).)

Related Product Information for anti-TK2 antibody

Thymidine kinase-2 (TK2) is a deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine, and also phosphorylates antiviral and anticancer nucleoside analogs. Both recombinant and native forms of the enzyme have broad substrate specificity and complex kinetics, suggesting that it may play a role in the activation of chemotherapeutic nucleoside analogs. The highest levels of expression are observed in testis and ovary. The main supply of deoxyribonucleotides (dNTPs) for mitochondrial DNA synthesis comes from the salvage pathway initiated by deoxyguanosine kinase (DGK) and TK2. The association of mitochondrial DNA depletion with mutations in the genes encoding these 2 kinases suggests that the salvage pathway enzymes are involved in the maintenance of balanced mitochondrial dNTP pools.

Product Categories/Family for anti-TK2 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 290656975
• NCBI GeneID: 7084
• NCBI Accession #: NP_001166114.1; NP_004605.4
• NCBI GenBank Nucleotide #: NM_001172643.1
• UniProt Accession #: O00142; O15238; B4DGJ7; B4DZK7; B7ZAB1; E9PH08
• Molecular Weight: 20,281 Da
• NCBI Official Full Name: thymidine kinase 2 isoform 2
• NCBI Official Synonym Full Names: thymidine kinase 2, mitochondrial
• NCBI Official Symbol: TK2
• NCBI Official Synonym Symbols: MTTK; SCA31; MTDPS2
• NCBI Protein Information: thymidine kinase 2; thymidine kinase 2, mitochondrial; mt-TK
• UniProt Protein Name: Thymidine kinase 2, mitochondrial
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK2
• UniProt Entry Name: KITM_HUMAN

NCBI Description

This gene encodes a deoxyribonucleoside kinase that specifically phosphorylates thymidine, deoxycytidine, and deoxyuridine. The encoded enzyme localizes to the mitochondria and is required for mitochondrial DNA synthesis. Mutations in this gene are associated with a myopathic form of mitochondrial DNA depletion syndrome. Alternate splicing results in multiple transcript variants encoding distinct isoforms, some of which lack transit peptide, so are not localized to mitochondria. [provided by RefSeq, Dec 2012]

Uniprot Description

TK2: Deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine. Also phosphorylates anti-viral and anti-cancer nucleoside analogs. Defects in TK2 are a cause of mitochondrial DNA depletion syndrome type 2 (MTDPS2). A disorder characterized primarily by childhood onset of muscle weakness associated with depletion of mtDNA in skeletal muscle. There is wide clinical variability; some patients have onset in infancy and show a rapidly progressive course with early death due to respiratory failure, whereas others have later onset of a slowly progressive myopathy. Belongs to the DCK/DGK family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; EC 2.7.1.21; Kinase, other; Mitochondrial; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 16q22-q23.1

Cellular Component: mitochondrial matrix; mitochondrial inner membrane

Molecular Function: deoxycytidine kinase activity; thymidine kinase activity; ATP binding

Biological Process: deoxycytidine metabolic process; pyrimidine base metabolic process; nucleotide biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; deoxyribonucleotide metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; mitochondrial DNA replication; phosphorylation; deoxyribonucleoside monophosphate biosynthetic process

Disease: Mitochondrial Dna Depletion Syndrome 2 (myopathic Type)