Typical Testing Data/Standard Curve (for reference only)
Mouse Thyroid transcription factor 1 ELISA Kit | TITF1 elisa kit
Mouse TITF1 (Thyroid transcription factor 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TITF1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TITF1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TITF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TITF1.You can calculate the concentration of TITF1in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
NKX2-1: Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC); also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). This syndrome include neurological, thyroid, and respiratory problems. Belongs to the NK-2 homeobox family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 14q13
Cellular Component: nucleoplasm; nucleus; transcription factor complex
Molecular Function: DNA binding; enzyme binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity
Biological Process: anatomical structure formation; axon guidance; brain development; cerebral cortex cell migration; cerebral cortex GABAergic interneuron differentiation; development of primary female sexual characteristics; endoderm development; feeding behavior; forebrain development; forebrain dorsal/ventral pattern formation; forebrain neuron fate commitment; globus pallidus development; hippocampus development; hyperosmotic salinity response; induction; Leydig cell differentiation; lung development; negative regulation of cell migration; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; neuron migration; oligodendrocyte differentiation; phospholipid metabolic process; pituitary gland development; positive regulation of circadian rhythm; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; renin-angiotensin regulation of blood volume; response to ethanol; response to hormone stimulus; response to lipopolysaccharide; rhythmic process; thyroid gland development; transcription, DNA-dependent
Disease: Chorea, Benign Hereditary; Choreoathetosis And Congenital Hypothyroidism With Or Without Pulmonary Dysfunction; Thyroid Carcinoma, Papillary
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Product Notes
The Mouse TITF1 nkx2-1 (Catalog #AAA2533481) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533481 ELISA Kit recognizes Mouse TITF1. It is sometimes possible for the material contained within the vial of "Thyroid transcription factor 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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