Rabbit anti-Human, Mouse NIPA4 Polyclonal Antibody | anti-NIPAL4 antibody

NIPA4 Antibody (N-term)

Cat. Num. AAA9209568

• Gene Names: NIPAL4; ARCI6; ICHYN; ICHTHYIN
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: NIPA4; Polyclonal Antibody; NIPA4 Antibody (N-term); Magnesium transporter NIPA4; Ichthyin; NIPA-like protein 4; Non-imprinted in Prader-Willi/Angelman syndrome region protein 4; NIPAL4; ICHN; anti-NIPAL4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This NIPA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-37 amino acids from the N-terminal region of human NIPA4.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 9-37
• Sequence Length: 447

• Applicable Applications for anti-NIPAL4 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100
• Function: Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Ba(2+), Mn(2+), Sr(2+) and Co(2+) but to a much less extent than Mg(2+) (By similarity). May be a receptor for ligands (trioxilins A3 and B3) from the hepoxilin pathway.
• Cellular Location: Membrane; Multi-pass membrane protein
• Tissue Location: Highly expressed in brain, lung, stomach, keratinocytes and leukocytes, and in all other tissues tested except liver, thyroid and fetal brain.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(NIPA4 Antibody (N-term) (MBS9209568) western blot analysis in mouse liver tissue lysates (15ug/lane). This demonstrates the NIPA4 antibody detected NIPA4 protein (arrow).)

Western Blot (WB)

(Anti-NIPA4 Antibody (N-term) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(NIPA4 antibody (N-term) (MBS9209568) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NIPA4 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-NIPAL4 antibody

Metabolism; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 149944536
• NCBI GeneID: 348938
• NCBI Accession #: NP_001092757.1; NP_001165763.1
• NCBI GenBank Nucleotide #: NM_001099287.1
• UniProt Accession #: Q0D2K0; Q0D2J8; Q0D2J9; A8S6F1; A8S6F5; A8S6F8; B4DLF3
• Molecular Weight: 50058
• NCBI Official Full Name: magnesium transporter NIPA4 isoform 1
• NCBI Official Synonym Full Names: NIPA-like domain containing 4
• NCBI Official Symbol: NIPAL4
• NCBI Official Synonym Symbols: ARCI6; ICHYN; ICHTHYIN
• NCBI Protein Information: magnesium transporter NIPA4
• UniProt Protein Name: Magnesium transporter NIPA4
• Protein Family: Magnesium transporter
• UniProt Gene Name: NIPAL4
• UniProt Synonym Gene Names: ICHN; NIPA4
• UniProt Entry Name: NIPA4_HUMAN

NCBI Description

This gene likely encodes a membrane receptor. Mutations in this gene have been associated with autosomal recessive congenital ichthyosis. [provided by RefSeq, Feb 2010]

Uniprot Description

ICHN: Acts as a Mg(2+) transporter. Can also transport other divalent cations such as Ba(2+), Mn(2+), Sr(2+) and Co(2+) but to a much less extent than Mg(2+). May be a receptor for ligands (trioxilins A3 and B3) from the hepoxilin pathway. Defects in NIPAL4 are the cause of ichthyosis congenital autosomal recessive ichthyin-related (ARCII). ARCII is a disorder of keratinization with abnormal differentiation and desquamation of the epidermis. The phenotype usually presents as non-bullous congenital ichthyosiform erythroderma (NCIE) with fine whitish scaling on an erythrodermal background; larger brownish scales are present on the buttocks, neck and legs. Some of the families may show a more lamellar phenotype (lamellar ichthyosis). Belongs to the NIPA family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane

Molecular Function: magnesium ion transmembrane transporter activity

Biological Process: magnesium ion transport

Disease: Ichthyosis, Congenital, Autosomal Recessive 6