Structure
(Typical Structure of an Antibody)
IgG2a, Rat Negative Control Isotype Control
IgG2a, Rat Negative Control
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
IgG2a; Rat Negative Control; Rat Negative Control isotype control
Host
Rat
Reactivity
Human
Isotype
IgG2a
Clone Number
3H2379
Specificity
Negative for all mouse cells and cell lines. Recognizes a human cell surface marker and therefore cannot be used as a negative control in this species.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid, 1% BSA, 0.1% sodium azide
Applicable Applications for IgG2a, Rat Negative Control isotype control
ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA.
Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by researcher.
Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by researcher.
Immunogen
Human lymphocytes.
Product Type
Mab
Hybridoma
Spleen cells from immunized DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Structure
(Typical Structure of an Antibody)
Structure
(Typical Structure of an Antibody)
Related Product Information for IgG2a, Rat Negative Control isotype control
Suitable for use as a rat IgG2a negative control for the measurement of non-specific binding of rat monoclonal antibodies of isotype IgG2a. Also suitable for use as a negative control with canine tissues. Negative on all mouse cells and cell lines tested. Recognizes human cell surface marker and therefore is not suitable for use as a negative control in this species.
Product Categories/Family for IgG2a, Rat Negative Control isotype control
Similar Products
Product Notes
The IgG2a, Rat Negative Control (Catalog #AAA655587) is an Isotype Control produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The IgG2a, Rat Negative Control reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG2a, Rat Negative Control can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the IgG2a, Rat Negative Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG2a, Rat Negative Control, Isotype Control" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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