Human IGFBP2 ELISA Kit | IGFBP2 elisa kit

Human IGFBP2 ELISA Kit

Cat. Num. AAA824653

• Gene Names: IGFBP2; IBP2; IGF-BP53
• Reactivity: Human
• Applications: ELISA
• Synonyms: IGFBP2; Human IGFBP2 ELISA Kit; BP2; IBP2; Insulin-like growth factor-binding protein 2; IBP-2; IGF-binding protein 2; IGFBP-2; IGFBP2 elisa kit

• Reactivity: Human
• Specificity: The Human IGFBP2 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IGFBP2 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 328

• Applicable Applications for IGFBP2 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma, Urine
• Sensitivity: The minimum detectable dose of Human IGFBP2 is typically less than 10 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP2 elisa kit

Principle of the assay: The Bioscience Human IGFBP2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IGFBP2 in Cell Culture Supernatants, Serum, Plasma, Urine. This assay employs an antibody specific for Human IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways is regulators of cancer-mediated endothelial recruitment.

NCBI and Uniprot Product Information

• NCBI GI #: 55925576
• NCBI GeneID: 3485
• NCBI Accession #: NP_000588.2
• NCBI GenBank Nucleotide #: NM_000597.2
• UniProt Accession #: P18065; Q14619; Q9UCL3
• Molecular Weight: 34,814 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 2
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 2, 36kDa
• NCBI Official Symbol: IGFBP2
• NCBI Official Synonym Symbols: IBP2; IGF-BP53
• NCBI Protein Information: insulin-like growth factor-binding protein 2; IBP-2; IGFBP-2; IGF-binding protein 2
• UniProt Protein Name: Insulin-like growth factor-binding protein 2
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP2
• UniProt Synonym Gene Names: BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP-2
• UniProt Entry Name: IBP2_HUMAN

Uniprot Description

IGFBP2: Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Binds IGF2 more than IGF1.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: extracellular space; apical plasma membrane; extracellular region; cytoplasmic vesicle

Molecular Function: protein binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: response to drug; regulation of insulin-like growth factor receptor signaling pathway; response to retinoic acid; response to lithium ion; response to glucocorticoid stimulus; female pregnancy; positive regulation of activated T cell proliferation; signal transduction; response to estradiol stimulus; cellular response to hormone stimulus; cellular protein metabolic process; response to mechanical stimulus; regulation of cell growth; response to nutrient; aging