TIMP2 / TIMP-2 recombinant protein

Recombinant Human TIMP2 / TIMP-2 Protein (Fc tag)

Cat. Num. AAA2545169

• Gene Names: TIMP2; DDC8; CSC-21K
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TIMP2 / TIMP-2; Recombinant Human TIMP2 / TIMP-2 Protein (Fc tag); CSC-21K; TIMP2; TIMP2 / TIMP-2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5
• Sequence Length: 220

• Application Notes: The recombinant human Fc/TIMP2 is a disulfide-linked homodimeric protein. The reduced monomer consists of 431 amino acids and has a predicted molecular mass of 48 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Glu 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TIMP2 / TIMP-2 recombinant protein

Background: Tissue inhibitors of metalloproteinases (TIMP) family are natural inhibitors of the matrix metalloproteinases (MMPs), the zinc enzymes involved in extracellular matrix maintenance and remodeling. The TIMP family encompasses four members (TIMP1-4), and they inhibit most MMPs by forming non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated protein expressed by a variety of cell type s, and plays a unique role among TIMP family members owing to its functions to regulate cellular responses to growth factors. Findings establish an unexpected, MMP-independent mechanism for TIMP2 inhibition of endothelial cell proliferation in vitro and reveal an important component of the antiangiogenic effect of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance of tissue homeostasis and is involved in the regulation of tumor microenvironment.

Description: A DNA sequence encoding the mature form of human TIMP2 (NP_003246.1) (Cys 27-Pro 220) was expressed with the fused Fc region of human IgG1 at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4507511
• NCBI GeneID: 7077
• NCBI Accession #: NP_003246.1
• NCBI GenBank Nucleotide #: NM_003255.4
• UniProt Accession #: P16035; Q16121; Q93006; Q9UDF7
• Molecular Weight: 24,399 Da
• NCBI Official Full Name: metalloproteinase inhibitor 2
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 2
• NCBI Official Symbol: TIMP2
• NCBI Official Synonym Symbols: DDC8; CSC-21K
• NCBI Protein Information: metalloproteinase inhibitor 2
• UniProt Protein Name: Metalloproteinase inhibitor 2
• UniProt Gene Name: TIMP2
• UniProt Synonym Gene Names: TIMP-2

NCBI Description

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]

Uniprot Description

TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Cell development/differentiation; Extracellular matrix; Inhibitor; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: protease binding; protein binding

Biological Process: extracellular matrix disassembly; negative regulation of membrane protein ectodomain proteolysis; response to cytokine stimulus; response to hormone stimulus