Rat TIMP-1 ELISA Kit | TIMP1 elisa kit

Rat TIMP-1 PicoKine ELISA Kit

Cat. Num. AAA175959

• Gene Names: Timp1; Timp; TIMP-1
• Reactivity: Rat
• Synonyms: TIMP-1; Rat TIMP-1 PicoKine ELISA Kit; Metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CCR5; CCR5_HUMAN; CD195; CHEMR13; CLGI; Collagenase inhibitor; EPA; EPO; Erythroid potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; HIV-1 fusion coreceptor; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP; TIMP 1; TIMP1 protein; Tissue Inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinases; Tissue inhibitor of metalloproteinases 1; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor; TIMP1 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat TIMP-1
• Sequence Length: 217

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 3pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat TIMP-1 PicoKine ELISA Kit standard curve )

Related Product Information for TIMP1 elisa kit

Principle of the assay: rat TIMP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TIMP-1 has been precoated onto 96-well plates. Standards (NSO,C24-A217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TIMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TIMP-1 amount of sample captured in plate.
Background: The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates humanand murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactivecomplexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard productused in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5KDa. As a result of glycosylation, the molecular mass is 32-34KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 1174697
• NCBI GeneID: 116510
• UniProt Accession #: P30120; P70533; Q53YM7
• Molecular Weight: 23,794 Da
• NCBI Official Full Name: Metalloproteinase inhibitor 1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1
• NCBI Official Symbol: Timp1
• NCBI Official Synonym Symbols: Timp; TIMP-1
• NCBI Protein Information: metalloproteinase inhibitor 1; tissue inhibitor of metallopeptidase 1; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinases 1
• UniProt Protein Name: Metalloproteinase inhibitor 1
• Protein Family: Metalloproteinase inhibitor
• UniProt Gene Name: Timp1
• UniProt Synonym Gene Names: Timp-1; TIMP-1
• UniProt Entry Name: TIMP1_RAT

NCBI Description

acts as an inhibitor of metalloprotease activity; may play a role in vascular tissue remodeling [RGD, Feb 2006]

Uniprot Description

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling. Also stimulates steroidogenesis by Leydig and ovarian granuloma cells; procathepsin L is required for maximal activity.