Toll Like Receptor Adaptor Molecule 1 Recombinant Protein | TICAM1 recombinant protein

Recombinant Toll Like Receptor Adaptor Molecule 1 (TICAM1)

Cat. Num. AAA2028960

• Gene Names: TICAM1; TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Toll Like Receptor Adaptor Molecule 1; Recombinant Toll Like Receptor Adaptor Molecule 1 (TICAM1); TICAM1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SLGLPHEI SVPEVSPEEA SPILPDALAA PDTSVHCPIE CTELSTNSRS PLTSTTESVG KQWPITSQRS PQVPVGDDSL QNTTSSSPPA QPPSLQASPK LPPSPLSSAS SPSSYPAPPT STSPVLDHSE TSDQKFYNFV VIHARADEQV ALRIREKLET LGVPDGATFC EEFQVPGRGE LHCLQDAIDH SGFTILLLTA SFDCSLSLHQ INHALMNSLT QSGRQDCVIP LLPLECSQAQ LSPDTTRLLH SIVWLDEHSPIFARKV
• Sequence Length: 712

• Applicable Applications for TICAM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 32.2kDa
• Accurate Molecular Mass (KD): 39kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met1~Glu135
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 223460864
• NCBI GeneID: 148022
• Molecular Weight: 76,422 Da
• NCBI Official Full Name: Toll-like receptor adaptor molecule 1
• NCBI Official Synonym Full Names: toll-like receptor adaptor molecule 1
• NCBI Official Symbol: TICAM1
• NCBI Official Synonym Symbols: TRIF; IIAE6; MyD88-3; PRVTIRB; TICAM-1
• NCBI Protein Information: TIR domain-containing adapter molecule 1
• UniProt Protein Name: TIR domain-containing adapter molecule 1
• Protein Family: TIR domain-containing adapter molecule
• UniProt Gene Name: TICAM1
• UniProt Synonym Gene Names: PRVTIRB; TRIF; TICAM-1; MyD88-3; TIR domain-containing adapter protein inducing IFN-beta
• UniProt Entry Name: TCAM1_HUMAN

NCBI Description

This gene encodes an adaptor protein containing a Toll/interleukin-1 receptor (TIR) homology domain, which is an intracellular signaling domain that mediates protein-protein interactions between the Toll-like receptors (TLRs) and signal-transduction components. This protein is involved in native immunity against invading pathogens. It specifically interacts with toll-like receptor 3, but not with other TLRs, and this association mediates dsRNA induction of interferon-beta through activation of nuclear factor kappa-B, during an antiviral immune response. [provided by RefSeq, Jan 2012]

Uniprot Description

TICAM1: an adaptor protein involved in innate immunity against invading pathogens. Associates with TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TICAM1 recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of the effector proteins TBK1, TRAF6 and RIPK1, which in turn, lead to the activation of transcription factors IRF3 and IRF7, NF-kappa-B and FADD respectively. Interacts with the TIR domain of TLR3. Interacts with AZI2, TBK1, IRF3 and IRF7. Interacts with TICAM2 in TLR4 recruitment. Interaction with PIAS4 inhibits the TICAM1-induced NF-kappa-B, IRF and IFNB1 activation. Interacts with IKBKB and IKBKE. Interaction with SARM1 blocks TICAM1-dependent transcription factor activation. Interacts with TRAF3. Interacts with TRAFD1.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: endosome membrane; cytosol

Molecular Function: signal transducer activity; protein binding; protein kinase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of nitric oxide biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of protein binding; regulation of protein homodimerization activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of interleukin-6 production; toll-like receptor 3 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of natural killer cell activation; activation of NF-kappaB transcription factor; positive regulation of chemokine biosynthetic process; response to exogenous dsRNA; positive regulation of protein ubiquitination; positive regulation of interferon-beta biosynthetic process; macrophage activation during immune response; positive regulation of B cell proliferation; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; innate immune response; inflammatory response; toll-like receptor 4 signaling pathway; defense response to virus

Disease: Herpes Simplex Encephalitis, Susceptibility To, 4