CD90 / THY-1 recombinant protein

Recombinant Rat CD90 / THY-1 Protein (His tag)

Cat. Num. AAA2547335

• Gene Names: Thy1; CD7
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CD90 / THY-1; Recombinant Rat CD90 / THY-1 Protein (His tag); THY1; Thy-1; CD90 / THY-1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 161

• Application Notes: The recombinant rat THY1 comprises 121 amino acids and predicts a molecular mass of 14.1 kDa. The apparent molecular mass of the recombinant protein is approximately 27-29 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Gln 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD90 / THY-1 recombinant protein

Background: Mouse Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype . Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.

Description: A DNA sequence encoding the rat THY1 (P01830) (Met1-Lys129) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 6981654
• NCBI GeneID: 24832
• NCBI Accession #: NP_036805.1
• NCBI GenBank Nucleotide #: NM_012673.2
• UniProt Accession #: P01830
• Molecular Weight: 18,172 Da
• NCBI Official Full Name: thy-1 membrane glycoprotein preproprotein
• NCBI Official Synonym Full Names: Thy-1 cell surface antigen
• NCBI Official Symbol: Thy1
• NCBI Official Synonym Symbols: CD7
• NCBI Protein Information: thy-1 membrane glycoprotein
• UniProt Protein Name: Thy-1 membrane glycoprotein
• UniProt Gene Name: Thy1
• UniProt Synonym Gene Names: Thy-1

NCBI Description

cell surface glycoprotein antigen [RGD, Feb 2006]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Cell adhesion; Membrane protein, GPI anchor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: anchored to external side of plasma membrane; anchored to plasma membrane; apical plasma membrane; cell surface; cytosol; dendrite; endoplasmic reticulum; external side of plasma membrane; focal adhesion; growth cone; lipid raft; myelin sheath; plasma membrane

Molecular Function: enzyme binding; GTPase activator activity; integrin binding; protein binding; protein kinase binding

Biological Process: angiogenesis; cell-cell adhesion; cytoskeleton organization and biogenesis; focal adhesion formation; negative regulation of apoptosis; negative regulation of axonogenesis; negative regulation of cell migration; negative regulation of protein kinase activity; negative regulation of T cell receptor signaling pathway; positive regulation of GTPase activity; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; protein amino acid autophosphorylation; retinal cone cell development; T cell receptor signaling pathway