Thrombospondin 3 Recombinant Protein | THBS3 recombinant protein

Recombinant Thrombospondin 3 (THBS3)

Cat. Num. AAA2031044

• Gene Names: THBS3; TSP3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Thrombospondin 3; Recombinant Thrombospondin 3 (THBS3); THBS3 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; D YVPPGPDNCR LVPNPNQKDS DGNGVGDVCE DDFDNDAVVD PLDVCPESAE VTLTDFRAYQ TVVLDPEGDA QIDPNWVVLN QGMEIVQTMN SDPGLAVGYT AFNGVDFEGT FHVNTVTDDD YAGFLFSYQD SGRFYVVMWK QTEQTYWQAT PFRAVAQPGL QLKAVTSVSG PGEHLRNALW HTGHTPDQVR LLWTDPRNVG WRDKTSYRWQLLHRPQVGYI RVKLYEGPQL VADSGVIIDT SMRGGRLGVF C
• Sequence Length: 836

• Applicable Applications for THBS3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 58.1kDa
• Accurate Molecular Mass (KD): 58kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, GST-tag, S-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Asp670~Cys921
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 357933596
• NCBI GeneID: 7059
• NCBI Accession #: NP_001239537.1
• NCBI GenBank Nucleotide #: NM_001252608.1
• Molecular Weight: 91,297 Da
• NCBI Official Full Name: thrombospondin-3 isoform 3
• NCBI Official Synonym Full Names: thrombospondin 3
• NCBI Official Symbol: THBS3
• NCBI Official Synonym Symbols: TSP3
• NCBI Protein Information: thrombospondin-3
• UniProt Protein Name: Thrombospondin-3
• Protein Family: Thrombospondin
• UniProt Gene Name: THBS3
• UniProt Synonym Gene Names: TSP3
• UniProt Entry Name: TSP3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants. [provided by RefSeq, Nov 2011]

Uniprot Description

THBS3: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. Belongs to the thrombospondin family.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: perinuclear region of cytoplasm; extracellular region

Molecular Function: heparin binding; calcium ion binding

Biological Process: cell-matrix adhesion