Probable queuine tRNA-ribosyltransferase (Tgt) Recombinant Protein | Tgt recombinant protein
Recombinant Drosophila melanogaster Probable queuine tRNA-ribosyltransferase (Tgt)
Gene Names
Tgt; CG4947; DmelCG4947; tgt; TGT
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Probable queuine tRNA-ribosyltransferase (Tgt); Recombinant Drosophila melanogaster Probable queuine tRNA-ribosyltransferase (Tgt); Tgt recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-427, Full length protein
Sequence
MGPSHIPPLTYKVVAECSVSKARAGLMTLRHSEVNTPVFMPVGTQGTLKGIVPDQLIELNCQILLGNTYHLGLRPGIETLKKAGGLHKFMGWPRAILTDSGGFQMVSLLQLAEIDEHGVNFRSPFDNSQCMLTPEHSIEIQNAIGGDIMMQLDDVVKTTTTGPRVEEAMERTIRWVDRCIEAHARDDDQSLFPIVQGGLDVPLRQRCVSALMERQVRGFAVGGLSGGESKHDFWRMVDVCTGYLPKDKPRYLMGVGFAADLVVCVALGIDMFDCVFPTRTARFGCALVDSGQLNLKQPKYKLDMEPIDKDCDCSTCRRYTRSYLHHIATNESVSSSLLSIHNVAYQLRLMRSMREAIQRDEFPQFVADFMARHFKAEPVPAWIREALSAVNIQLPADPERIDEQDQKPKTEKRRETEDVAEEQVASS
Sequence Length
427
Species
Drosophila melanogaster (Fruit fly)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,751 Da
NCBI Official Full Name
tRNA-guanine transglycosylase
NCBI Official Synonym Full Names
tRNA-guanine transglycosylase
NCBI Official Symbol
Tgt
NCBI Official Synonym Symbols
CG4947; DmelCG4947; tgt; TGT
NCBI Protein Information
CG4947 gene product from transcript CG4947-RA
UniProt Protein Name
Queuine tRNA-ribosyltransferase catalytic subunit
Protein Family
UniProt Gene Name
Tgt
Uniprot Description
Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product.
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Product Notes
The Tgt tgt (Catalog #AAA1298036) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-427, Full length protein. The amino acid sequence is listed below: MGPSHIPPLT YKVVAECSVS KARAGLMTLR HSEVNTPVFM PVGTQGTLKG IVPDQLIELN CQILLGNTYH LGLRPGIETL KKAGGLHKFM GWPRAILTDS GGFQMVSLLQ LAEIDEHGVN FRSPFDNSQC MLTPEHSIEI QNAIGGDIMM QLDDVVKTTT TGPRVEEAME RTIRWVDRCI EAHARDDDQS LFPIVQGGLD VPLRQRCVSA LMERQVRGFA VGGLSGGESK HDFWRMVDVC TGYLPKDKPR YLMGVGFAAD LVVCVALGID MFDCVFPTRT ARFGCALVDS GQLNLKQPKY KLDMEPIDKD CDCSTCRRYT RSYLHHIATN ESVSSSLLSI HNVAYQLRLM RSMREAIQRD EFPQFVADFM ARHFKAEPVP AWIREALSAV NIQLPADPER IDEQDQKPKT EKRRETEDVA EEQVASS. It is sometimes possible for the material contained within the vial of "Probable queuine tRNA-ribosyltransferase (Tgt), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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