Transforming growth factor beta-1 (Tgfb1) Recombinant Protein | Tgfb1 recombinant protein

Recombinant Rat Transforming growth factor beta-1 (Tgfb1), partial

Cat. Num. AAA717883

• Gene Names: Tgfb1; Tgfb
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Transforming growth factor beta-1 (Tgfb1); Recombinant Rat Transforming growth factor beta-1 (Tgfb1); partial; Cleaved into the following chain: 1. Latency-associated peptide; 2. LAP; Tgfb1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 30-278. Partial
• Sequence: LSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESADPEPEPEADYYAKEVTRVLMVDRNNAIYDKTKDITHSIYMFFNTSDIREAVPEPPLLSRAELRLQRFKSTVEQHVELYQKYSNNSWRYLGNRLLTPTDTPEWLSFDVTGVVRQWLNQGDGIQGFRFSAHCSCDSKDNVLHVEINGISPKRRGDLGTIHDMNRPFLLLMATPLERAQHLHSSRHRR

• Species: Rat
• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for Tgfb1 recombinant protein

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells.

Product Categories/Family for Tgfb1 recombinant protein

Signal Transduction

References

"cDNA cloning by PCR of rat transforming growth factor beta-1." Qian S.W., Kondaiah P., Roberts A.B., Sporn M.B. Nucleic Acids Res. 18:3059-3059(1990)

NCBI and Uniprot Product Information

• NCBI GI #: 11024652
• NCBI GeneID: 59086
• NCBI Accession #: NP_067589.1
• NCBI GenBank Nucleotide #: NM_021578.2
• UniProt Accession #: P17246; Q53YM8
• Molecular Weight: 30.7
• NCBI Official Full Name: transforming growth factor beta-1
• NCBI Official Synonym Full Names: transforming growth factor, beta 1
• NCBI Official Symbol: Tgfb1
• NCBI Official Synonym Symbols: Tgfb
• NCBI Protein Information: transforming growth factor beta-1
• UniProt Protein Name: Transforming growth factor beta-1
• Protein Family: Transforming growth factor
• UniProt Gene Name: Tgfb1
• UniProt Synonym Gene Names: TGF-beta-1; LAP
• UniProt Entry Name: TGFB1_RAT

NCBI Description

binds the TGFbeta receptor; plays a role in regulation of cell growth and proliferation; induces synthesis of extracellular matrix proteins and may play a role in fibrosis [RGD, Feb 2006]

Uniprot Description

TGFB1: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3. May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with CD109, DPT and ASPN. Activated in vitro at pH below 3.5 and over 12.5. Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Belongs to the TGF-beta family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Cellular Component: axon; cell soma; cell surface; cytoplasm; extracellular matrix; extracellular space; microvillus; nucleus; proteinaceous extracellular matrix; secretory granule

Molecular Function: antigen binding; cytokine activity; enzyme binding; glycoprotein binding; protein heterodimerization activity; protein homodimerization activity; protein N-terminus binding; protein serine/threonine kinase activator activity; punt binding; transforming growth factor beta receptor binding

Biological Process: activation of NF-kappaB transcription factor; adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains; aging; ATP biosynthetic process; cell activation; cell cycle arrest; cell migration; cell proliferation; cellular calcium ion homeostasis; chondrocyte differentiation; common-partner SMAD protein phosphorylation; defense response to fungus, incompatible interaction; endoderm development; epidermal growth factor receptor signaling pathway; epithelial to mesenchymal transition; evasion of host defenses by virus; female pregnancy; germ cell migration; gut development; heart development; hemopoietic progenitor cell differentiation; hyaluronan catabolic process; inflammatory response; inner ear development; intercellular junction assembly and maintenance; lipopolysaccharide-mediated signaling pathway; lung development; lymph node development; mammary gland development; MAPKKK cascade; membrane protein intracellular domain proteolysis; mitotic cell cycle checkpoint; mononuclear cell proliferation; morphogenesis of a branching structure; myelination; myeloid dendritic cell differentiation; negative regulation of blood vessel endothelial cell migration; negative regulation of cell cycle; negative regulation of cell differentiation; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cell-cell adhesion; negative regulation of DNA replication; negative regulation of epithelial cell proliferation; negative regulation of fat cell differentiation; negative regulation of immune response; negative regulation of interleukin-17 production; negative regulation of mitotic cell cycle; negative regulation of myoblast differentiation; negative regulation of neuroblast proliferation; negative regulation of ossification; negative regulation of phagocytosis; negative regulation of protein amino acid phosphorylation; negative regulation of release of sequestered calcium ion into cytosol; negative regulation of skeletal muscle development; negative regulation of T cell activation; negative regulation of T cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube closure; neural tube development; Notch signaling pathway; oligodendrocyte development; organ regeneration; phosphate metabolic process; positive regulation of apoptosis; positive regulation of blood vessel endothelial cell migration; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cellular protein metabolic process; positive regulation of chemotaxis; positive regulation of collagen biosynthetic process; positive regulation of epithelial cell proliferation; positive regulation of exit from mitosis; positive regulation of fibroblast proliferation; positive regulation of histone acetylation; positive regulation of histone deacetylation; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgA isotypes; positive regulation of MAP kinase activity; positive regulation of odontogenesis; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase activity; positive regulation of protein amino acid dephosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of protein complex assembly; positive regulation of protein import into nucleus; positive regulation of protein kinase B signaling cascade; positive regulation of protein secretion; positive regulation of regulatory T cell differentiation; positive regulation of smooth muscle cell differentiation; positive regulation of superoxide release; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vascular permeability; protein amino acid phosphorylation; protein export from nucleus; protein import into nucleus, translocation; protein kinase B signaling cascade; receptor catabolic process; regulation of binding; regulation of cell growth; regulation of cell proliferation; regulation of DNA binding; regulation of gene expression; regulation of interleukin-23 production; regulation of MAPKKK cascade; regulation of protein import into nucleus; regulation of regulatory T cell differentiation; regulation of sodium ion transport; regulation of striated muscle development; regulation of transforming growth factor beta receptor signaling pathway; regulatory T cell differentiation; response to drug; response to estradiol stimulus; response to glucose stimulus; response to hypoxia; response to organic cyclic substance; response to organic substance; response to progesterone stimulus; response to radiation; response to vitamin D; response to wounding; salivary gland morphogenesis; SMAD protein complex assembly; SMAD protein nuclear translocation; T cell activation; T cell differentiation; T cell homeostasis; tolerance induction to self antigen; transforming growth factor beta receptor signaling pathway; ureteric bud development; vasculogenesis; ventricular cardiac muscle morphogenesis; wound healing