Monkey Transferrin Receptor Protein 2 ELISA Kit | TFR2 elisa kit

Monkey Transferrin Receptor Protein 2 ELISA Kit

Cat. Num. AAA737101

• Gene Names: TFR2; HFE3; TFRC2
• Reactivity: Monkey
• Synonyms: Transferrin Receptor Protein 2; Monkey Transferrin Receptor Protein 2 ELISA Kit; TFR2 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFR2 elisa kit

Principle of the Assay: TFR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFR2 antibody and an TFR2-HRP conjugate. The assay sample and buffer are incubated together with TFR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFR2 concentration since TFR2 from samples and TFR2-HRP conjugate compete for the anti-TFR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TFR2 from the sample, fewer sites are left to bind TFR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFR2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This TFR2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TFR2. This ELISA kit for research use only!

Product Categories/Family for TFR2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 332309171
• NCBI GeneID: 7036
• NCBI Accession #: NP_001193784.1
• NCBI GenBank Nucleotide #: NM_001206855.1
• Molecular Weight: 86,012 Da
• NCBI Official Full Name: transferrin receptor protein 2 isoform 2
• NCBI Official Synonym Full Names: transferrin receptor 2
• NCBI Official Symbol: TFR2
• NCBI Official Synonym Symbols: HFE3; TFRC2
• NCBI Protein Information: transferrin receptor protein 2
• UniProt Protein Name: Transferrin receptor protein 2
• Protein Family: Transferrin receptor protein
• UniProt Gene Name: TFR2
• UniProt Synonym Gene Names: TfR2
• UniProt Entry Name: TFR2_HUMAN

NCBI Description

This gene encodes a single-pass type II membrane protein, which is a member of the transferrin receptor-like family. This protein mediates cellular uptake of transferrin-bound iron, and may be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Mutations in this gene have been associated with hereditary hemochromatosis type III. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011]

Uniprot Description

TFR2: Mediates cellular uptake of transferrin-bound iron in a non-iron dependent manner. May be involved in iron metabolism, hepatocyte function and erythrocyte differentiation. Defects in TFR2 are a cause of hemochromatosis type 3 (HFE3). HFE3 is a disorder of iron hemostasis resulting in iron overload and has a phenotype indistinguishable from that of hereditary hemochromatosis (HH). HH is characterized by abnormal intestinal iron absorption and progressive increase of total body iron, which results in midlife in clinical complications including cirrhosis, cardiopathy, diabetes, endocrine dysfunctions, arthropathy, and susceptibility to liver cancer. Since the disease complications can be effectively prevented by regular phlebotomies, early diagnosis is most important to provide a normal life expectancy to the affected subjects. Belongs to the peptidase M28 family. M28B subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: integral to plasma membrane; cytoplasm

Molecular Function: transferrin receptor activity

Biological Process: receptor-mediated endocytosis; cellular iron ion homeostasis; iron ion transport

Disease: Hemochromatosis, Type 3