Typical Testing Data/Standard Curve (for reference only)
Mouse Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit | GSTa3 elisa kit
Mouse Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit
Gene Names
Gsta3; Gst2-3
Reactivity
Mouse
Synonyms
Glutathione S Transferase Alpha 3 (GSTa3); Mouse Glutathione S Transferase Alpha 3 (GSTa3) ELISA Kit; GST-A3; GSTA3-3; GTA3; GST class-alpha member 3; GSTa3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of GSTa3. No significant cross-reactivity or interference between GSTa3 and analogues was observed.
Sequence Length
221
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
<0.049ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GSTa3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GSTa3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GSTa3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of GSTa3 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSTa3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GSTa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSTa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GSTa3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GSTa3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GSTa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GSTa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GSTa3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,361 Da
NCBI Official Full Name
glutathione S-transferase A3 isoform a
NCBI Official Synonym Full Names
glutathione S-transferase, alpha 3
NCBI Official Symbol
Gsta3
NCBI Official Synonym Symbols
Gst2-3
NCBI Protein Information
glutathione S-transferase A3
UniProt Protein Name
Glutathione S-transferase A3
Protein Family
UniProt Gene Name
Gsta3
UniProt Synonym Gene Names
Gstyc
Uniprot Description
Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This GST has a high catalytic activity for aflatoxin B1-8,9 epoxide.
Research Articles on GSTa3
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Product Notes
The Mouse GSTa3 gsta3 (Catalog #AAA457041) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457041 ELISA Kit recognizes Mouse GSTa3. It is sometimes possible for the material contained within the vial of "Glutathione S Transferase Alpha 3 (GSTa3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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