Trefoil Factor-3 Recombinant Protein | rTFF3 recombinant protein

Recombinant Rat Trefoil Factor-3

Cat. Num. AAA145662

• Gene Names: Tff3; ITF
• Purity: Purity as determined by densitometric image analysis is greater than 95%.
• Synonyms: Trefoil Factor-3; Recombinant Rat Trefoil Factor-3; TFF3 Rat; Trefoil Factor-3 Rat Recombinant; Trefoil factor 3; Intestinal trefoil factor; rITF; Polypeptide P1.B; rP1.B; Tff3; Itf; rTFF3 recombinant protein

• Host: E Coli
• Purity/Purification: Purity as determined by densitometric image analysis is greater than 95%.
• Form/Format: TFF3 protein filtered (0.4 um) and lyophilized in 0.5mg/ml in 20mM TRIS and 50mM NaCl, pH 7.5.; Sterile Filtered White lyophilized (freeze-dried) powder.
• Sequence: MQEFVGLSPS QCMVPANVRV DCGYPTVTSE QCNNRGCCFD SSIPNVPWCF KPLQETECT F DYKDDDDK
• Sequence Length: 81

• Solubility: It is recommended to add 200 ul deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

Related Product Information for rTFF3 recombinant protein

Description: The Trefoil Factor-3 Rat was constructed as a recombinant protein with a 9 a.a C-terminal fusion of DYKDDDDK-tag (1 aa N-terminal+8 aa C-terminal). The TFF3 Rat produced in E Coli, is 7.7kDa protein containing a total of 68 amino acid residues.

Introduction: Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.

Product Categories/Family for rTFF3 recombinant protein

Cytokines and Growth Factors; Cytokines; Trefoil Factor

NCBI and Uniprot Product Information

• NCBI GI #: 386869333
• NCBI GeneID: 25563
• NCBI Accession #: NP_037174.2
• NCBI GenBank Nucleotide #: NM_013042.2
• UniProt Accession #: Q03191; Q68FZ2
• Molecular Weight: 8,944 Da
• NCBI Official Full Name: trefoil factor 3
• NCBI Official Synonym Full Names: trefoil factor 3, intestinal
• NCBI Official Symbol: Tff3
• NCBI Official Synonym Symbols: ITF
• NCBI Protein Information: trefoil factor 3; intestinal trefoil factor; polypeptide P1.B
• UniProt Protein Name: Trefoil factor 3
• UniProt Gene Name: Tff3
• UniProt Synonym Gene Names: Itf; rITF; rP1.B
• UniProt Entry Name: TFF3_RAT

NCBI Description

plays a role in epithelial wound healing; involved in maintenance of the intestinal mucosa [RGD, Feb 2006]

Uniprot Description

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) ().