Methylcytosine dioxygenase TET1 (Tet1) Recombinant Protein | Tet1 recombinant protein

Recombinant Mouse Methylcytosine dioxygenase TET1 (Tet1) , partial

Cat. Num. AAA1290203

• Gene Names: Tet1; LCX; Cxxc6; AA517754; BB001228; mKIAA1676; D10Ertd17e; 2510010B09Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Methylcytosine dioxygenase TET1 (Tet1); Recombinant Mouse Methylcytosine dioxygenase TET1 (Tet1); partial; Tet1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1528-1931aa; Partial
• Sequence: SWSMYFNGCKFGRSENPRKFRLAPNYPLHEKQLEKNLQELATVLAPLYKQMAPVAYQNQVEYEEVAGDCRLGNEEGRPFSGVTCCMDFCAHSHKDIHNMHNGSTVVCTLIRADGRDTNCPEDEQLHVLPLYRLADTDEFGSVEGMKAKIKSGAIQVNGPTRKRRLRFTEPVPRCGKRAKMKQNHNKSGSHNTKSFSSASSTSHLVKDESTDFCPLQASSAETSTCTYSKTASGGFAETSSILHCTMPSGAHSGANAAAGECTGTVQPAEVAAHPHQSLPTADSPVHAEPLTSPSEQLTSNQSNQQLPLLSNSQKLASCQVEDERHPEADEPQHPEDDNLPQLDEFWSDSEEIYADPSFGGVAIAPIHGSVLIECARKELHATTSLRSPKRGVPFRVSLVFYQHK

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 241896976
• NCBI GeneID: 52463
• NCBI Accession #: NP_081660.1
• NCBI GenBank Nucleotide #: NM_027384.1
• UniProt Accession #: Q3URK3; Q6ZPK2; Q9CY36
• Molecular Weight: 219,261 Da
• NCBI Official Synonym Full Names: tet methylcytosine dioxygenase 1
• NCBI Official Symbol: Tet1
• NCBI Official Synonym Symbols: LCX; Cxxc6; AA517754; BB001228; mKIAA1676; D10Ertd17e; 2510010B09Rik
• NCBI Protein Information: methylcytosine dioxygenase TET1
• UniProt Protein Name: Methylcytosine dioxygenase TET1
• Protein Family: Methylcytosine dioxygenase
• UniProt Gene Name: Tet1
• UniProt Synonym Gene Names: Cxxc6; Kiaa1676

Uniprot Description

Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).